Solution‐State Structure of a Long‐Loop G‐Quadruplex Formed Within Promoters of Plasmodium falciparum B var Genes

Juribašić Kulcsar, Marina; Gabelica, Valérie; Plavec, Janez (2024) Solution‐State Structure of a Long‐Loop G‐Quadruplex Formed Within Promoters of Plasmodium falciparum B var Genes. Chemistry – A European Journal, 30 (36). ISSN 0947-6539

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Abstract

We report the high-resolution NMR solution-state structure of an intramolecular G-quadruplex with a diagonal loop of ten nucleotides. The G-quadruplex is formed by a 34-nt DNA sequence, d[CAG3T2A2G3TATA2CT3AG4T2AG3T2], named UpsB-Q-1. This sequence is found within promoters of the var genes of Plasmodium falciparum, which play a key role in malaria pathogenesis and evasion of the immune system. The [3+1]-hybrid G-quadruplex formed under physiologically relevant conditions exhibits a unique equilibrium between two structures, both stabilized by base stacking and non-canonical hydrogen bonding. Unique equilibrium of the two closely related 3D structures originates from a North-South repuckering of deoxyribose moiety of residue T27 in the lateral loop. Besides the 12 guanines involved in three G-quartets, most residues in loop regions are involved in interactions at both G-quartet-loop interfaces.

Item Type:	Article
Uncontrolled Keywords:	G-quadruplex ; NMR spectroscopy ; structure determination ; solution ; Plasmodium falciparum
Subjects:	NATURAL SCIENCES > Chemistry
Divisions:	Division of Physical Chemistry
Depositing User:	Ana Zečević
Date Deposited:	23 Jul 2024 07:29
URI:	http://fulir.irb.hr/id/eprint/8964
DOI:	10.1002/chem.202401190

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