Identification and analysis of cDNAs encoding two nucleoside diphosphate kinases (NDPK/Nm23) from the marine sponge Suberites domuncula

Harcet, Matija; Lukić-Bilela, Lada; Ćetković, Helena; Müller, E.G. Werner; Gamulin, Vera (2005) Identification and analysis of cDNAs encoding two nucleoside diphosphate kinases (NDPK/Nm23) from the marine sponge Suberites domuncula. Croatica Chemica Acta, 78 (3). pp. 343-348. ISSN 0011-1643

PDF - Published Version
Download (276kB)

Official URL: http://hrcak.srce.hr/index.php?show=clanak&id_clan...

Abstract

Suberites domuncula is a member of the most ancient and simplest extant phylum of multicellular animals - sponges (Porifera). A database of S. domuncula expressed sequence tags (ESTs) was recently constructed by random cDNA sequencing. Two NDPK/Nm23 proteins from the sponge Suberites domuncula are reported here. Sponge proteins were named Nm23-SD1 and Nm23-SD6, because they display the highest sequence similarity with human Nm23-H1 and -H6 proteins. Overall sequence conservation of Nm23-SD1 with human Nm23-H1 is very high -79% (71% identical amino acids). Nm23-SD6 possesses an insertion at the C-terminus and displays 55% overall homology (40% identical amino acids) with human Nm23-H6. Secondary structure predictions for both sponge and human Nm23 protein pairs are almost identical. S. domuncula Nm23 proteins display high similarity to homologues from mammals/humans, higher than to e.g. NDPK/Nm23 proteins from Drosophila or other invertebrates. Sponge Nm23 proteins are more similar to mammalian/human Nm23 proteins than most known Nm23 proteins of invertebrates.

Item Type:

Article

Uncontrolled Keywords:

antimetastatic proteins; nucleoside diphosphate kinases; sponge cDNA library; Porifera; secondary structure prediction; inhibitory-activity; nm23; cells; colonization; expression; carcinoma; metastasis; invasion; sequence

Subjects:

NATURAL SCIENCES > Chemistry

Divisions:

Division of Molecular Biology

Projects: