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Application of Long-Chained Auxin Conjugates Influenced Auxin Metabolism and Transcriptome Response in Brassica rapa L. ssp. pekinensis

Smolko, Ana; Repar, Jelena; Matković, Marija; Pavlović, Iva; Penčik, Aleš; Novák, Ondřej; Ludwig-Müller, Jutta; Salopek-Sondi, Branka (2023) Application of Long-Chained Auxin Conjugates Influenced Auxin Metabolism and Transcriptome Response in Brassica rapa L. ssp. pekinensis. International Journal of Molecular Sciences, 25 (1). ISSN 1422-0067

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Auxin amino acid conjugates are considered to be storage forms of auxins. Previous research has shown that indole-3-acetyl-L-alanine (IAA-Ala), indole-3-propionyl-L-alanine (IPA-Ala) and indole-3-butyryl-L-alanine (IBA-Ala) affect the root growth of Brassica rapa seedlings. To elucidate the potential mechanism of action of the conjugates, we treated B. rapa seedlings with 0.01 mM IAA-, IPA- and IBA-Ala and investigated their effects on the auxin metabolome and transcriptome. IBA-Ala and IPA-Ala caused a significant inhibition of root growth and a decrease in free IAA compared to the control and IAA-Ala treatments. The identification of free auxins IBA and IPA after feeding experiments with IBA-Ala and IPA-Ala, respectively, confirms their hydrolysis in vivo and indicates active auxins responsible for a stronger inhibition of root growth. IBA-Ala caused the induction of most DEGs (807) compared to IPA-Ala (417) and IAA-Ala (371). All treatments caused similar trends in transcription profile changes when compared to control treatments. The majority of auxin-related DEGs were found after IBA-Ala treatment, followed by IPA-Ala and IAA-Ala, which is consistent with the apparent root morphology. In addition to most YUC genes, which showed a tendency to be downregulated, transcripts of auxin-related DEGs that were identified (UGT74E2, GH3.2, SAUR, IAA2, etc.) were more highly expressed after all treatments. Our results are consistent with the hypothesis that the hydrolysis of conjugates and the release of free auxins are responsible for the effects of conjugate treatments. In conclusion, free auxins released by the hydrolysis of all auxin conjugates applied affect gene regulation, auxin homeostasis and ultimately root growth inhibition.

Item Type: Article
Uncontrolled Keywords: amino acid auxin conjugates; Brassica rapa; indole-3-acetic acid; indole-3-butyric acid; indole-3-propionic acid; auxin metabolome; root growth inhibition; transcriptome
NATURAL SCIENCES > Biology > Biochemistry and Molecular Biology
Divisions: Division of Molecular Biology
Project titleProject leaderProject codeProject type
Fitohormoni u abiotskom stresu kupusnjača: mehanizam tolerancije i primjena-PhytoBraCroBranka Salopek-SondiIP-2014-09-4359HRZZ
Depositing User: Branka Salopek Sondi
Date Deposited: 08 Feb 2024 13:06
DOI: 10.3390/ijms25010447

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