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Potentiometric Investigation of Specific Ionic Effects on Interactions between Bovine Serum Albumin and Weak Polyelectrolytes

Mutka, Saniela; Njegić Džakula, Branka; Kovačević, Davor (2008) Potentiometric Investigation of Specific Ionic Effects on Interactions between Bovine Serum Albumin and Weak Polyelectrolytes. Croatica Chemica Acta, 81 (4). pp. 593-597. ISSN 0011-1643

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Abstract

The effect of salt on the behaviour of bovine serum albumin (BSA) in solution and on the interactions between BSA and the weakly charged polyelectrolytes poly(allylamine hydrochloride) and poly(dimethylaminoethylmethacrylate) was investigated by potentiometric titrations. Titrations of pure BSA solution and of the BSA solution with the addition of polyelectrolyte were performed in the presence of different salts. Three electrolytes having the same anion and a different cation were used. lithium chloride, sodium chloride, and caesium chloride. The corresponding base served as titrant. Experiments were performed at four electrolyte concentrations. The titration curves showed a strong influence of ionic strength and the electrolyte type. The cation effect was found to be in accordance with the Hofmeister series.

Item Type: Article
Uncontrolled Keywords: potentiometric titration; bovine serum albumin; weak polyelectrolytes; ionic strength; multilayers; complexation; copolymers; mechanism; films
Subjects: NATURAL SCIENCES > Chemistry
Divisions: Division of Materials Chemistry
Projects:
Project titleProject leaderProject codeProject type
Fizikalna kemija koloida i međupovršina[19896] Nikola Kallay119-1191342-2961MZOS
Mehanizmi taloženja anorganskih biokompatibilnih i srodnih materijala[120470] Damir Kralj098-0982904-2951MZOS
Depositing User: Branka Njegić Džakula
Date Deposited: 22 Oct 2013 11:14
Last Modified: 14 Feb 2014 14:28
URI: http://fulir.irb.hr/id/eprint/821

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