Maleš, Petra; Brkljača, Zlatko; Domazet Jurašin, Darija; Bakarić, Danijela (2023) New Spirit of an Old Technique: Characterization of Lipid Phase Transitions via UV/Vis Spectroscopy. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 272 . ISSN 1386-1425
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Abstract
One of the advantages of investigating lipid phase transitions by thermoanalytical techniques such as DSC is manifested in the proportionality of the signal strength on a DSC curve, attributed to a particular thermotropic event, and its cooperativity degree. Accordingly, the pretransition of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) is less noticeable than its main phase transition; as a matter of fact, when DSC measurements are performed at low heating rate, such low-cooperativity phase transition could go (almost) unnoticed. The aim of this work is to present temperature-dependent UV/Vis spectroscopy, based on a temperature-dependent change in DPPC suspension turbidity, as a technique applicable for determination of lipid phase transition temperatures. Multivariate analyzes of the acquired UV/Vis spectra show that phase transitions of the low-cooperativity degree, such as pretransitions, can be identified with the same certainty as transitions of a high-cooperativity degree.
| Item Type: | Article | ||||||||
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| Uncontrolled Keywords: | UV/Vis spectroscopy; 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC); cholesterol; lipid phase transitions; differential spectral analysis (DSA); multivariate curve analysis (MCA) | ||||||||
| Subjects: | NATURAL SCIENCES > Chemistry > Physical Chemistry | ||||||||
| Divisions: | Division of Organic Chemistry and Biochemistry Division of Physical Chemistry |
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| Depositing User: | Danijela Bakarić | ||||||||
| Date Deposited: | 27 Oct 2023 11:00 | ||||||||
| URI: | http://fulir.irb.hr/id/eprint/8190 | ||||||||
| DOI: | 10.1016/j.saa.2022.121013 |
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