New Spirit of an Old Technique: Characterization of Lipid Phase Transitions via UV/Vis Spectroscopy

Maleš, Petra; Brkljača, Zlatko; Domazet Jurašin, Darija; Bakarić, Danijela (2023) New Spirit of an Old Technique: Characterization of Lipid Phase Transitions via UV/Vis Spectroscopy. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 272 . ISSN 1386-1425

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Abstract

One of the advantages of investigating lipid phase transitions by thermoanalytical techniques such as DSC is manifested in the proportionality of the signal strength on a DSC curve, attributed to a particular thermotropic event, and its cooperativity degree. Accordingly, the pretransition of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) is less noticeable than its main phase transition; as a matter of fact, when DSC measurements are performed at low heating rate, such low-cooperativity phase transition could go (almost) unnoticed. The aim of this work is to present temperature-dependent UV/Vis spectroscopy, based on a temperature-dependent change in DPPC suspension turbidity, as a technique applicable for determination of lipid phase transition temperatures. Multivariate analyzes of the acquired UV/Vis spectra show that phase transitions of the low-cooperativity degree, such as pretransitions, can be identified with the same certainty as transitions of a high-cooperativity degree.

Item Type:

Article

Uncontrolled Keywords:

UV/Vis spectroscopy; 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC); cholesterol; lipid phase transitions; differential spectral analysis (DSA); multivariate curve analysis (MCA)

Subjects:

NATURAL SCIENCES > Chemistry > Physical Chemistry

Divisions:

Division of Organic Chemistry and Biochemistry
Division of Physical Chemistry

Projects: