Pašalić, Lea; Liu, Qiqian; Vukosav, Petra; Mišić-Radić, Tea; Azziz, Aicha; Majdinasa, Marjan; Edely, Mathieu; de la Chapelle, Mary Lamy; Bakarić, Danijela (2023) The Presence of Uncoated Gold Nanoparticle Aggregates May Alter the Phase of Phosphatidylcholine Lipid as Evidenced by Vibrational Spectroscopies. Journal of Liposome Research . ISSN 0898-2104
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Abstract
Spherical structures built from uni- and multilamellar lipid bilayers (LUV and MLV) are nowadays considered not just as nanocarriers of various kinds of therapeutics, but also as the vehicles that, when coupled with gold (Au) nanoparticles (NPs), can also serve as a tool for imaging and discriminating healthy and diseased tissues. Since the presence of Au NPs or their aggregates may affect the properties of the drug delivery vehicle, we investigated how the shape and position of Au NP aggregates adsorbed on the surface of MLV affect the arrangement and conformation of lipid molecules. By preparing MLVs constituted from 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) in the presence of uncoated Au NP aggregates found i) both within liposome core and on the surface of the outer lipid bilayer, or ii) adsorbed on the outer lipid bilayer surface only, we demonstrated the maintenance of lipid bilayer integrity by microscopic techniques (cryo-TEM, and AFM). The employment of SERS and FTIR-ATR tehniques enabled us not only to elucidate the lipid interaction pattern and their orientation in regards to Au NP aggregates, but also unequivocally confirmed the impact of Au NP aggregates on persistence/breaking of van der Waals interactions between hydrocarbon chains of DPPC.
| Item Type: | Article | ||||||||
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| Uncontrolled Keywords: | 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC); Gold nanoparticle (Au NP) aggregates; interaction pattern and lipid phase change; microscopy (cryo-EM, TEM, AFM), spectroscopy (SERS and FTIR-ATR) and calorimetry | ||||||||
| Subjects: | NATURAL SCIENCES > Chemistry > Physical Chemistry | ||||||||
| Divisions: | Division for Marine and Enviromental Research Division of Organic Chemistry and Biochemistry |
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| Depositing User: | Danijela Bakarić | ||||||||
| Date Deposited: | 30 Nov 2023 14:46 | ||||||||
| URI: | http://fulir.irb.hr/id/eprint/8189 | ||||||||
| DOI: | 10.1080/08982104.2023.2239905 |
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