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Morpho-physiological adaptations of Leptocylindrus aporus and L. hargravesii to phosphate limitation in the northern Adriatic

Kužat, Nataša; Smodlaka Tanković, Mirta; Baričević, Ana; Ivančić, Ingrid; Vrana Špoljarić, Ivna; Gašparović, Blaženka; Marić Pfannkuchen, Daniela; Pfannkuchen, Martin (2022) Morpho-physiological adaptations of Leptocylindrus aporus and L. hargravesii to phosphate limitation in the northern Adriatic. Scientific Reports, 12 . ISSN 2045-2322

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The northern Adriatic is highly productive and shallow area characterized by numerous spatio temporal gradients (e.g. nutrients, salinity, temperature). It is strongly infuenced by numerous freshwater inputs, mainly from Po river. Its current systems as well as Po river, generates gradients of phosphate availability with an expressed N/P imbalance and phosphate limitation. A number of recent studies characterized these gradients as major factors afecting abundance and composition of microphytoplankton communities. Focus of this study is on two Leptocylindrus species, Leptocylindrus aporus (F.W. French & Hargraves) D. Nanjappa & A. Zingone 2013 and Leptocylindrus hargravesii D. Nanjappa & A. Zingone 2013. Species belonging to Leptocylindrus genus are frequently observed and have high abundances and also high contributions to the microphytoplankton community in this area. We focused on their morphological and physiological responses to phosphate limitation in situ and also performed in vitro experiments. In this study we report data on species specifc growth rates under phosphorus (P) deplete and P rich conditions, localization and characteristics of alkaline phosphate activity, phosphate uptake rates as well as their morphological diferences in P deplete versus P rich conditions. Our in vitro experiments showed that both Leptocylindrus species morphologically reacted similarly to phosphorus depletion and showed signifcantly elongated pervalvar axis in P depleted conditions if compared to P rich conditions. Also average chain lengths increased when in P depleted conditions. Two previously mentioned adaptations indicate their tendency to increase cellular surface areas available for alkaline phosphatase. Chlorophyll fuorescence of both species signifcantly decreased in P depleted medium. Although both species morphologically reacted similarly, our experiment demonstrated signifcant diferences in physiological reactions to P depleted conditions.

Item Type: Article
Uncontrolled Keywords: Leptocylindrus aporus ; L. hargravesii ; phosphate limitation ; northern Adriatic
Subjects: NATURAL SCIENCES > Interdisciplinary Natural Sciences
NATURAL SCIENCES > Interdisciplinary Natural Sciences > Marine Science
Divisions: Center for Marine Research
Project titleProject leaderProject codeProject type
Životne strategije fitoplanktona u sjevernom Jadranu-P-limitMartin PfannkuchenUIP-2014-09-6563HRZZ
Molekularne životne strategije fitoplanktona u Jadranu-Adri LIFEDaniela Marić PfannkuchenUIP-2020-02-7868HRZZ
Utjecaj okolišnog stresa na pojavnost i međudjelovanje biološki važnih organskih molekula i mikronutrijenata u morskom ekosustavu-AMBIOMERESBlaženka GašparovićIP-2013-11-8607HRZZ
Depositing User: Blaženka Gašparović
Date Deposited: 05 Oct 2023 11:30
DOI: 10.1038/s41598-022-06062-5

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