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Hyposalinity induces significant polar lipid remodeling in the marine microalga Dunaliella tertiolecta (Chlorophyceae)

Vrana Špoljarić, Ivna; Bakija Alempijević, Saranda; Novosel, Nives; Ivošević DeNardis, Nadica; Žigon, Dušan; Ogrinc, Nives; Gašparović, Blaženka (2022) Hyposalinity induces significant polar lipid remodeling in the marine microalga Dunaliella tertiolecta (Chlorophyceae). Journal of Applied Phycology, 34 . pp. 1457-1470. ISSN 0921-8971

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Abstract

Marine microalgae often live in a fluctuating environment including a decrease in salinity caused by global warming induced sea ice melting and freshwater inflows. Under conditions of fluctuating salinity, microalgae have evolved a variety of survival mechanisms such as lipid accumulation and remodeling. The purpose of this study was to investigated the membrane lipid remodeling of the marine green microalga Dunaliella tertiolecta as a short-term acclimation mechanism in response to hyposalinity (20 and 3 PSU) with respect to growth at optimal salinity (38 PSU). We identified 34 lipid species belonging to seven polar lipid classes. Dunaliella tertiolecta accumulates cell lipids and remodels polar lipid classes and their fatty acids composition as response to hypoosmotic stress at 3 PSU. We found that the unsaturation of most polar lipids decreases overall, indicating decreased membrane fluidity and altered permeability, whereas shortening the length of fatty acids of polar lipids is not one of the strategies of D. tertiolecta to cope with the decrease in salinity. Increase in relative content (%) and unsaturation of monogalactosyldiacylglycerols (MGDG) and decrease in relative content (%) and unsaturation of phosphatidylglycerols (PG), suggesting changes in photosynthetic membranes of thylakoids at 20 and 3 PSU. At a very low salinity of 3 PSU, the relative content (%) of phosphatidylinositols (PI) increases, suggesting increased lipid trafficking and signaling in the cells. These changes are statistically significant and we hypothesize that D. tertiolecta is genetically adapted to withstand large salinity fluctuations through polar lipid composition.

Item Type: Article
Uncontrolled Keywords: hyposaline ; Dunaliella tertiolecta ; lipid remodeling ; polar lipids ; fatty acids
Subjects: NATURAL SCIENCES > Interdisciplinary Natural Sciences
NATURAL SCIENCES > Interdisciplinary Natural Sciences > Marine Science
Divisions: Division for Marine and Enviromental Research
Projects:
Project titleProject leaderProject codeProject type
Od površinskih svojstava stanica alga do pokazatelja stresa u vodenim ekosustavima-CELLSTRESSNadica Ivošević DeNardisIP-2018-01-5840HRZZ
Utjecaj okolišnog stresa na pojavnost i međudjelovanje biološki važnih organskih molekula i mikronutrijenata u morskom ekosustavu-AMBIOMERESBlaženka GašparovićIP-2013-11-8607HRZZ
Depositing User: Blaženka Gašparović
Date Deposited: 05 Oct 2023 11:09
URI: http://fulir.irb.hr/id/eprint/8150
DOI: 10.1007/s10811-022-02745-8

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