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The 4-Hydroxynonenal–Protein Adducts and Their Biological Relevance: Are Some Proteins Preferred Targets?

Milković, Lidija; Žarković, Neven; Marušić, Zlatko; Žarković, Kamelija; Jaganjac, Morana (2023) The 4-Hydroxynonenal–Protein Adducts and Their Biological Relevance: Are Some Proteins Preferred Targets?. Antioxidants, 12 (4). ISSN 2076-3921

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It is well known that oxidative stress and lipid peroxidation (LPO) play a role in physiology and pathology. The most studied LPO product with pleiotropic capabilities is 4-hydroxynonenal (4-HNE). It is considered as an important mediator of cellular signaling processes and a second messenger of reactive oxygen species. The effects of 4-HNE are mainly attributed to its adduction with proteins. Whereas the Michael adducts thus formed are preferred in an order of potency of cysteine > histidine > lysine over Schiff base formation, it is not known which proteins are the preferred targets for 4-HNE under what physiological or pathological conditions. In this review, we briefly discuss the methods used to identify 4-HNE–protein adducts, the progress of mass spectrometry in deciphering the specific protein targets, and their biological relevance, focusing on the role of 4-HNE protein adducts in the adaptive response through modulation of the NRF2/KEAP1 pathway and ferroptosis.

Item Type: Article
Uncontrolled Keywords: lipid peroxidation ; 4-hydroxynonenal (4-HNE) ; 4-HNE–protein adducts ; immunochemical methods ; mass spectrometry (MS) ; adaptive response ; the NRF2/KEAP1 signaling ; ferroptosis
Subjects: NATURAL SCIENCES > Biology
Divisions: Division of Molecular Medicine
Depositing User: Neven Žarković
Date Deposited: 23 Jun 2023 10:38
DOI: 10.3390/antiox12040856

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