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Methylation of Immune Gene Promoters in Oral and Oropharyngeal Cancer

Anić, Petra; Golubić Talić, Jasminka; Božinović, Ksenija; Dediol, Emil; Mravak-Stipetić, Marinka; Grce, Magdalena; Milutin Gašperov, Nina (2023) Methylation of Immune Gene Promoters in Oral and Oropharyngeal Cancer. International Journal of Molecular Sciences, 24 (9). ISSN 1422-0067

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The proportion of oral and oropharyngeal squamous cell carcinoma (OOSCC) that can be attributed to human papillomavirus (HPV) infection is growing nowadays. A potential factor indicating the occurrence of HPV-positive OSCC is a change in the degree of methylation of gene promoters that play a key role in the immune response. In this study, we investigated the difference in the methylation of EDARADD, GBP4, HAVCR2, HLA DPB1, IL12RB1, MARCO, and SIGLEC12 gene promoters in samples of healthy oral mucosa versus samples of oral and oropharyngeal cancer. The presence of HPV infection in samples was examined earlier. To determine the difference in methylation of those gene promotors, isolated and bisulfite-modified DNA was analysed by the methylation-specific PCR method. The investigated gene promoters were found to be more hypomethylated in the oral and oropharyngeal cancer samples in comparison to normal tissue. The proportion of unmethylated gene promoters was similar in HPV-positive and HPV- negative cancers, although the data should be confirmed on a larger set of samples. To conclude, in samples of healthy oral mucosa, the investigated gene promoters were found to be methylated in a high percentage (73.3% to 100%), while in oral and oropharyngeal cancer samples, they were methylated in a low percentage (11.1% to 37%), regardless of HPV infection.

Item Type: Article
Uncontrolled Keywords: oral and oropharyngeal cancer ; immune system genes ; DNA methylation ; HPV
Subjects: BIOMEDICINE AND HEALTHCARE > Basic Medical Sciences
BIOMEDICINE AND HEALTHCARE > Clinical Medical Sciences
Divisions: Division of Molecular Biology
Division of Molecular Medicine
Project titleProject leaderProject codeProject type
Epigenetičke promjene karcinoma pločastih stanica glave i vrata-EpiC-HNSCCMagdalena GrceIP-2013-11-4758HRZZ
Depositing User: Nina Milutin Gašperov
Date Deposited: 21 Jun 2023 12:41
DOI: 10.3390/ijms24097698

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