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Chromatographic and spectrophometric studies of vanadate (+V) reduction by 3–mercaptopropionic acid

Bura-Nakić, Elvira; Knežević, Lucija; Mandić, Jelena (2022) Chromatographic and spectrophometric studies of vanadate (+V) reduction by 3–mercaptopropionic acid. Journal of inorganic biochemistry, 230 . ISSN 0162-0134

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The reduction of vanadate (+V) in the presence of 3-mercaptopropionic acid was studied using a chromatographic method for the determination of vanadate (+V) versus vanadyl (+IV) species. Ion chromatography was combined with spectrophotometric investigation of the absorption properties of the solution. The chromatographic method for the separation of vanadate (+V) and vanadyl (+IV) was carried out with an anion exchange column. In the initial reaction mixture containing vanadate (+V) and 3-mercaptopropionic acid, ethylenediaminetetraacetic acid – EDTA was added in an excessive amount relative to the concentration of reactants in the solution. After the ligand exchange reaction, the added EDTA terminates the reduction, allowing redox speciation in the solutions. A strong pH dependence of the reduction rates in the investigated solution was observed. The vanadate reduction seems to proceed in 2 steps: 1) formation of the intermediate vanadate (+V)- thioester ; 2) reduction reaction and formation of the vanadyl (+IV)-thiol complex. The obtained results strongly suggest that the reaction of vanadate (+V)-thioester formation is proton catalyzed. It was observed that the overall reduction rates are pH dependent due to the complex vanadate (+V) solution speciation and changes in the ionic form of 3-mercaptopropionic acid.

Item Type: Article
Uncontrolled Keywords: Vanadate; Reduction; Thiols; Spectrophotometry; Ion-chromatography
Divisions: Division for Marine and Enviromental Research
Project titleProject leaderProject codeProject type
Geokemija i zapis redoks indikatora u različitim uvjetima okoliša: prema boljem razumijevanju redoks uvjeta u prošlostiBura-Nakić, ElviraIP-2018-01-7813HRZZ
Depositing User: Lucija Knežević
Date Deposited: 19 Apr 2023 10:50
DOI: 10.1016/j.jinorgbio.2022.111747

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