hrvatski jezikClear Cookie - decide language by browser settings

Reconstructed membrane vesicles from the microalga Dunaliella as a potential drug delivery system

Levak, Maja; Demir-Yilmaz, Irem; Formosa-Dague, Cécile; Vrana Špoljarić, Ivna; Gašparović, Blaženka; Horvat, Lucija; Butorac, Ana; Frkanec, Ruža; Ivošević DeNarids, Nadica (2023) Reconstructed membrane vesicles from the microalga Dunaliella as a potential drug delivery system. Bioelectrochemsitry, 150 . ISSN 1567-5394

PDF - Published Version - article
Available under License Creative Commons Attribution.

Download (10MB) | Preview


The aim of this biophysical study is to characterize reconstructed membrane vesicles obtained from microalgae in terms of their morphology, properties, composition, and ability to transport a model drug. The reconstructed vesicles were either emptied or non-emptied and exhibited a non-uniform distribution of spherical surface structures that could be associated with surface coat proteins, while in between there were pore-like structures of up to 10 nm that could contribute to permeability. The reconstructed vesicles were very soft and hydrophilic, which could be attributed to their composition. The vesicles were rich in proteins and were mostly derived from the cytoplasm and chloroplasts. We demonstrated that all lipid classes of D. tertiolecta are involved in the formation of the reconstructed membrane vesicles, where they play fundamental role to maintain the vesicle structure. The vesicles appeared to be permeable to calcein, impermeable to FITC-ovalbumin, and semipermeable to FITC-concanavalin A, which may be due to a specific surface interaction with glucose/mannose units that could serve as a basis for the development of drug carriers. Finally, the reconstructed membrane vesicles could pave a new way as sustainable and environmentally friendly marine bioinspired carriers and serve for studies on microtransport of materials and membrane-related processes contributing to advances in life sciences and biotechnology.

Item Type: Article
Uncontrolled Keywords: Drug delivery, Membrane vesicle, Microalgae, Nanomechanics, Permeability
NATURAL SCIENCES > Interdisciplinary Natural Sciences > Marine Science
NATURAL SCIENCES > Interdisciplinary Natural Sciences > Environmental Science
Divisions: Division for Marine and Enviromental Research
Project titleProject leaderProject codeProject type
Od površinskih svojstava stanica alga do pokazatelja stresa u vodenim ekosustavima-CELLSTRESSNadica Ivošević DeNardisIP-2018-01-5840HRZZ
Evaluation of the biophysical and physiological effects of salinity stress on algal cells and its consequences for cell aggregationNadica Ivošević Denardis Cecile Formosa DagueUNSPECIFIEDUNSPECIFIED
Depositing User: Nadica Ivošević DeNardis
Date Deposited: 06 Mar 2023 12:59
DOI: 10.1016/j.bioelechem.2022.108360

Actions (login required)

View Item View Item


Downloads per month over past year

Increase Font
Decrease Font
Dyslexic Font