Novel arsenic hyper-resistant bacteria from an extreme environment, Crven Dol mine, Allchar, North Macedonia

Bermanec, Vladimir; Paradžik, Tina; Kazazić, Snježana P.; Venter, Chantelle; Hrenović, Jasna; Vujaklija, Dušica; Duran, Robert; Boev, Ivan; Boev, Blažo (2021) Novel arsenic hyper-resistant bacteria from an extreme environment, Crven Dol mine, Allchar, North Macedonia. Journal of hazardous materials, 402 . ISSN 0304-3894

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Abstract

Novel hyper-resistant bacteria were isolated from the Crven Dol mine (Allchar, North Macedonia), arsenic-rich extreme environment. Bacteria were recovered from a secondary mineral mixture, an alteration of hydrothermal realgar rich in arsenates (pharmacolite, hornesite, and talmessite). The sample was recovered from the dark part of the mine at 28 m depth. Three bacterial strains and a bacterial consortium were isolated for their capacity to survive exposure to 32 g/L (209 mM) of arsenite, and 176 g/L (564 mM) of arsenate. The 16S rRNA gene analysis identified bacterial isolates as Stenotrophomonas sp. and two Microbacterium spp. This analysis also revealed that bacterial consortium comprise two Bacteriodetes exhibiting similarity to Olivibacter ginsengisoli and to uncultured bacterium, and one γ-proteobacteria with similarity to Luteimonas sp. Among all isolates Stenotrophomonas sp. exhibited the highest tolerance to As compound as well as the capacity to accumulate As inside the cells. Analysis of genes involved in As-resistance showed that recovered isolates possess the genes encoding the ArsB, Acr3(1) and Acr3(2) proteins, indicating that at least a part of their resistance could be ascribed to As-efflux systems described in isolates obtained from human-polluted environments.

Item Type:	Article
Uncontrolled Keywords:	Biogeochemistry ; Arsenate ; Arsenite ; Hyper As-resistance ; As-transporters ; 16S rRNA
Subjects:	NATURAL SCIENCES > Biology NATURAL SCIENCES > Biology > Microbiology NATURAL SCIENCES > Biology > Ecology
Divisions:	Division of Physical Chemistry
Projects:	Project title Project leader Project code Project type Bioprospecting Jadranskog mora Čož-Rakovac, Rozelinda; Dragović-Uzelac, Verica; Šantek, Božidar; Jokić, Stela; Jerković, Igor; Kraljević Pavelić, Sandra KK.01.1.1.01.0002 EK Prirodno stanište klinički značajnih Acinetobacter baumannii Hrenović, Jasna IP-2014-09-5656 HRZZ
Depositing User:	Dušica Vujaklija
Date Deposited:	07 Dec 2022 14:11
URI:	http://fulir.irb.hr/id/eprint/7716
DOI:	10.1016/j.jhazmat.2020.123437

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