Application of MCR-ALS with EFA on FT-IR Spectra of Lipid Bilayers in the Assessment of Phase Transition Temperatures: Potential for Discernment of Coupled Events

Maleš, Petra; Brkljača, Zlatko; Crnolatac, Ivo; Bakarić, Danijela (2022) Application of MCR-ALS with EFA on FT-IR Spectra of Lipid Bilayers in the Assessment of Phase Transition Temperatures: Potential for Discernment of Coupled Events. Colloids and surfaces. B, Biointerfaces, 201 . ISSN 0927-7765

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Abstract

Elucidation of the origin of the ripple phase displayed by multibilayers constituted from phosphatidylcholine lipids is a rather challenging task from both experimental and computational perspective. Despite apparent supremacy of various structural techniques in characterizing the periodic undulations, the interpretation of their results remains ambiguous. The application of FT-IR spectroscopy in temperature-dependent regime, along with appropriate spectral treatment and analysis, may enable deciphering the events that drive ripple phase formation on a molecular level. Temperature-dependent transmission FT-IR spectra, along with DSC measurements, were conducted on lipid multibilayers constituted from 1,2-dipalmitoyl-sn-glycero-3-phosphocholine. Lipid multibilayers made from 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine, which do not form a ripple phase, were examined as a reference. Spectra were analyzed using multivariate curve resolution technique with alternating least squares and evolving factor analysis (MCR-ALS with EFA) and lipid phase transition temperatures were determined. Polar parts of lipid molecules exert greater response on a ripple phase formation than non-polar ones. However, vibrational signatures of hydrocarbon chains that originate from intramolecular or combined intra- and intermolecular contributions display certain qualitative differences that pave the way for future work oriented on uncoupling the events that drive ripple phase formation.

Item Type:	Article
Uncontrolled Keywords:	1,2-Dipalmitoyl-sn-glycero-3-phosphocholine (DPPC); 1,2-Dipalmitoyl-sn-glycero-3-phosphoethanolamine (DPPE); Transmission FT-IR spectra, Differential scanning calorimetry; Multivariate curve resolution method; Phase transition(s) temperature(s) assessment; Ripple phase
Subjects:	NATURAL SCIENCES > Chemistry > Physical Chemistry
Divisions:	Division of Organic Chemistry and Biochemistry
Depositing User:	Danijela Bakarić
Date Deposited:	01 Dec 2022 09:12
URI:	http://fulir.irb.hr/id/eprint/7637
DOI:	10.1016/j.colsurfb.2021.111645

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