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Shewanella spp. from wastewater treatment plant‑afected environment: isolation and characterization

Topić Popović, Natalija; Kazazić, Snježana; Bilić, Branka; Babić, Sanja; Bojanić, Krunoslav; Bujak, Maro; Tartaro Bujak, Ivana; Jadan, Margita; Strunjak-Perović, Ivančica; Kepec, Slavko; Čož- Rakovac, Rozelinda (2022) Shewanella spp. from wastewater treatment plant‑afected environment: isolation and characterization. Environmental science and pollution research . pp. 1-18. ISSN 0944-1344

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Bacteria from the genus Shewanella are inhabitants of marine and freshwater ecosystems, recognized fish spoilage bacteria, but less known as fish disease agents. Shewanella spp. isolated from fish living in waters close to effluents of a wastewater treatment plant (WWTP) were not previously characterized. We have tested Shewanella isolates from WWTP-affected waters and related fish. Genotypic characterization identified most strains as S. baltica and S. oneidensis. In order to investigate the sensibility and accuracy of their MALDI-TOF MS identification, they were grown on two culture media enriched by various NaCl concentrations, incubated at different temperatures and duration. We analyzed their antimicrobial susceptibility on a panel of antimicrobial drugs and capacity for biofilm production. With a view to demonstrate their capacity to produce fatty acids, we assessed the impact of different culture media on their lipid profile. We performed zebrafish embryotoxicity tests to simulate the environmental infection of the earliest life stages in S. baltica-contaminated waters. The best MALDI-TOF MS identification scores were for strains cultivated on TSA for 24 h at 22 °C and with supplementation of 1.5% NaCl. Less than 17% of isolates demonstrated antimicrobial resistance. Most isolates were weak biofilm producers. Strain-to- strain variation of MIC and MBC was low. The major fatty acids were C15:0, C16:0, C16:1, C17:1, and iC15:0. Exposure of Danio rerio to different S. baltica concentrations induced severe effects on zebrafish development: decreased heartbeat rate, locomotor activity, and melanin pigmentation. S. baltica passed through chorionic pores of zebrafish.

Item Type: Article
Uncontrolled Keywords: Antimicrobial resistance ; Biofilm ; Fatty acid profile ; Identification ; MALDI-TOF MS ; Shewanella ; WWTP ; Zebrafish
Subjects: NATURAL SCIENCES > Biology
BIOMEDICINE AND HEALTHCARE > Veterinary Medicine > Basic and Preclinical Veterinary Sciences
Divisions: Division of Materials Chemistry
Division of Physical Chemistry
Project titleProject leaderProject codeProject type
Bioprospecting Jadranskog mora-Rozelinda Čož-Rakovac; Verica Dragović-Uzelac; Božidar Šantek; Stela Jokić; Igor Jerković; Sandra Kraljević PavelićKK.
Depositing User: Natalija Topić Popović
Date Deposited: 25 Aug 2022 09:00
DOI: 10.1007/s11356-022-21573-4

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