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Metal-binding biomolecules in the liver of northern pike (Esox lucius Linnaeus, 1758): the first data for the family Esocidae

Dragun, Zrinka; Ivanković, Dušica; Krasnići, Nesrete; Kiralj, Zoran; Cvitanović, Marita; Valić, Damir; Karamatić, Ivana; Barac, Fran; Filipović Marijić, Vlatka; Mijošek, Tatjana; Gjurčević, Emil; Matanović, Krešimir; Kužir, Snježana (2022) Metal-binding biomolecules in the liver of northern pike (Esox lucius Linnaeus, 1758): the first data for the family Esocidae. Comparative Biochemistry and Physiology - Part C: Toxicology and Pharmacology, 257 . ISSN 1532-0456

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Abstract

Metal-handling strategies of various fish species are known to vary significantly in association with their intracellular metal behaviour. Thus, to better understand the possible consequences of increased metal exposure in fish it is important to perform comparative studies on metal binding biomolecules in organs of different species. This study was the first of this kind on a liver of an esocid fish (northern pike, Esox lucius), and the gathered information were compared to fish belonging to three other families, Leuciscidae, Cyprinidae and Salmonidae. Distributions of ten elements among cytosolic biomolecules of different molecular masses were studied by size exclusion HPLC combined offline with high resolution ICP-MS. The results indicated predominant association of Co, Fe and Mo to high molecular mass biomolecules (>100 kDa), of Zn and Bi to both high and medium molecular mass biomolecules (>30 kDa), of Mn and Se to medium molecular mass biomolecules (30-100 kDa), and Ag, Cd and Cu to low molecular mass biomolecules (10-30 kDa), presumably metallothioneins. Evident binding to metallothioneins was also detected for Zn and Bi. For several metals, distinct differences were observed when cytosolic metal distributions of northern pike were compared to leuciscids, salmonids and cyprinids. More pronounced Zn binding to metallothioneins was recorded in leuciscids and cyprinids than both esocids and salmonids, whereas cytosolic Mn and Se distributions clearly differed between all studied fish families. Accordingly, in assessment of metal pollution it is vital to consider the exposed species, which requires prior comprehensive comparative research on numerous aquatic organisms.

Item Type: Article
Uncontrolled Keywords: cytosol ; freshwater fish ; green liver syndrome ; HPLC ; ICP-MS
Subjects: NATURAL SCIENCES > Chemistry
NATURAL SCIENCES > Chemistry > Analytic Chemistry
NATURAL SCIENCES > Biology
NATURAL SCIENCES > Interdisciplinary Natural Sciences
Divisions: Division for Marine and Enviromental Research
Projects:
Project titleProject leaderProject codeProject type
Biomolekule koje vežu metale i zdravstveni poremećaji kod slatkovodnih organizama izloženih industrijskom otpadu-METABIOMZrinka DragunIP-2019-04-2636HRZZ
Depositing User: Zrinka Dragun
Date Deposited: 31 Mar 2022 12:34
URI: http://fulir.irb.hr/id/eprint/7093
DOI: 10.1016/j.cbpc.2022.109327

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