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Bioprospecting of Less-Polar Fractions of Ericaria crinita and Ericaria amentacea: Developmental Toxicity and Antioxidant Activity

Radman, Sanja; Čižmek, Lara; Babić, Sanja; Cikoš, Ana-Marija; Čož-Rakovac, Rozelindra; Jokić, Stela; Jerković, Igor (2021) Bioprospecting of Less-Polar Fractions of Ericaria crinita and Ericaria amentacea: Developmental Toxicity and Antioxidant Activity. Marine drugs, 20 (1). ISSN 1660-3397

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Ericaria crinita and Ericaria amentacea from the Adriatic Sea (Croatia) were investigated with respect to the presence of less-polar compounds for the first time after fractionation by solid-phase extraction (SPE). The composition of less-polar fractions of freeze-dried E. crinita (FdEc) and E. amentacea (FdEa) were analyzed by high- performance liquid chromatography–high-resolution mass spectrometry with electrospray ionization (UHPLC-ESI-HRMS). The major identified compounds were: amides of higher aliphatic acids (palmitoleamide, linoleamide, palmitamide, oleamide and erucamide) and related compounds, carotenoid (fucoxanthin), chlorophyll derivatives (pheophytin a and b and their derivatives) and higher terpenes (loliolide, isoamijiol with its oxidation product), β-stigmasterol and (3β, 6α)-14- methylergosta-8, 24(28)-diene-3, 6-diol). The toxic effects observed on the less-polar fractions obtained from Ericaria species on zebrafish Danio rerio embryos could be associated with the high abundance of all five detected amides. The antioxidant activity of the fractions was evaluated by means of five independent assays, including the reduction of the radical cation (ABTS), the oxygen radical absorbance capacity (ORAC), ferric-reducing antioxidant power (FRAP), the 2, 2-diphenyl-1-picryl-hydrazyl (DPPH) assay and the Folin–Ciocalteu method. A higher antioxidant activity of E. amentacea in comparison to that of the E. crinita fractions was found with IC50 concentrations of 0.072 and 1.177 mg/mL, respectively. The correlation between the activity and the chemical composition revealed that the synergistic effect of different compounds impacted their antioxidant response.

Item Type: Article
Uncontrolled Keywords: brown algae ; UHPLC-ESI-HRMS ; fatty acid amides ; fucoxanthin ; pheophytin a, b and their derivatives ; terpenes ; zebrafish embryotoxicity ; antioxidant activity
Subjects: BIOTECHNICAL SCIENCES > Biotechnology
Divisions: Division of Materials Chemistry
Project titleProject leaderProject codeProject type
Bioprospecting Jadranskog mora-Rozelinda Čož-Rakovac; Verica Dragović-Uzelac; Božidar Šantek; Stela Jokić; Igor Jerković; Sandra Kraljević PavelićKK.
Depositing User: Kristina Ciglar
Date Deposited: 17 Jan 2022 10:03
DOI: 10.3390/md20010057

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