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Microbiome of the Successful Freshwater Invader, the Signal Crayfish, and Its Changes along the Invasion Range

Dragičević, Paula; Bielen, Ana; Petrić, Ines; Vuk, Marija; Žučko, Jurica; Hudina, Sandra (2021) Microbiome of the Successful Freshwater Invader, the Signal Crayfish, and Its Changes along the Invasion Range. Microbiology Spectrum, 9 (2). ISSN 2165-0497

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Abstract

Increasing evidence denotes the role of the microbiome in biological invasions, since it is known that microbes can affect the fitness of the host. Here, we demonstrate differences in the composition of an invader’s microbiome along the invasion range, suggesting that its microbial communities may affect and be affected by range expansion. Using a 16S rRNA gene amplicon sequencing approach, we (i) analyzed the microbiomes of different tissues (exoskeleton, hemolymph, hepatopancreas, and intestine) of a successful freshwater invader, the signal crayfish, (ii) compared them to the surrounding water and sediment, and (iii) explored their changes along the invasion range. Exoskeletal, hepatopancreatic, and intestinal microbiomes varied between invasion core and invasion front populations. This indicates that they may be partly determined by population density, which was higher in the invasion core than in the invasion front. The highly diverse microbiome of exoskeletal biofilm was partly shaped by the environment (due to the similarity with the sediment microbiome) and partly by intrinsic crayfish parameters (due to the high proportion of exoskeleton- unique amplicon sequence variants [ASVs]), including the differences in invasion core and front population structure. Hemolymph had the most distinct microbiome compared to other tissues and differed between upstream (rural) and downstream (urban) river sections, indicating that its microbiome is potentially more driven by the effects of the abiotic environment. Our findings offer an insight into microbiome changes during dispersal of a successful invader and present a baseline for assessment of their contribution to an invader’s overall health and its further invasion success.

Item Type: Article
Uncontrolled Keywords: invasive species ; Pacifastacus leniusculus ; 16S rRNA gene ; microbiome ; range expansion
Subjects: NATURAL SCIENCES > Biology
Divisions: Division for Marine and Enviromental Research
Projects:
Project titleProject leaderProject codeProject type
Promjene sastava patogena i imunološkog odgovora tijekom širenja areala uspješnih invazivnih vrsta slatkovodnih rakovaHudina, SandraUIP-2017-05-1720HRZZ
Depositing User: Ines Petrić
Date Deposited: 31 Dec 2021 10:17
URI: http://fulir.irb.hr/id/eprint/6861
DOI: 10.1128/Spectrum.00389-21

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