Association between aryl hydrocarbon receptor and 4-hydroxynonenal in oxidative stress-mediated chronic rhinosinusitis with nasal polyps

Kvarantan, Aigerim; Balta, Vedran; Žarković, Neven; Horvat, Tea; Vuković, Tea; Žarković, Kamelija; Kalogjera, Livije (2021) Association between aryl hydrocarbon receptor and 4-hydroxynonenal in oxidative stress-mediated chronic rhinosinusitis with nasal polyps. European Journal of Inflammation, 11 (12). ISSN 1721-727X

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Abstract

Objective: Сhronic rhinosinusitis with nasal polyps (CRSwNPs) is a distinct entity within the chronic rhinosinusitis group of diseases, which are chronic upper airway diseases with several pheno- and endotypes. Oxidative stress plays an important role in the pathogenesis of CRSwNPs. The aim was to assess the association between expression of the aryl hydrocarbon receptor (AhR) and 4-hydroxynonenal (4-HNE) in patients with CRSwNPs. Methods: The study included 26 patients who underwent endoscopic sinus surgery: 14 patients with CRSwNPs, and 12 controls with healthy sinus mucosa. Expression of AhR and 4-HNE was assessed in tissue samples using immunohistochemistry. The level of 4-HNE in serum samples was measured using the ELISA assay. Total oxidative capacity (TOC) was assessed by measuring the peroxidase activity. Results: Higher levels of 4-HNE expression were observed in tissues (3, range 1-3 vs. 0, range 0-0 p<0.001) and serum (27.7±11.5 vs. 9.8±7.7 pmol/mg, p < 0.001) samples of CRSwNPs patients, as compared to healthy controls. Higher expression of AhR was found in inflammatory cells (plasma cells, lymphocytes, eosinopholes) of CRSwNPs patients, compared to controls (3, range 1-3 vs. 2, range 1- 2, p = 0.001). There were no differences in TOC across groups (0.0285±0.0207 vs. 0.02978±0.0197 µM H2O2 eq., p=0.848). Patients with bronchial asthma (57%) had abundant eosinophiles in tissue samples. Patients with recalcitrant CRSwNPs had higher 4- HNE serum levels, compared to non-recalcitrant cases (27.3 vs. 24.2 pmol/mg, p=0.339). Conclusion: Patients suffering from CRSwNPs have oxidative stress mediated overexpression of AhR, which is linked to a chronic inflammatory response in the paranasal sinus tissues.

Item Type:	Article
Uncontrolled Keywords:	4-hydroxynonenal ; aryl hydrocarbon receptor ; chronic rhinosinusitis ; oxidative stress ; reactive oxygen species
Subjects:	BIOMEDICINE AND HEALTHCARE > Clinical Medical Sciences
Divisions:	Division of Molecular Medicine
Depositing User:	Sofija Konjević
Date Deposited:	30 Dec 2021 12:30
URI:	http://fulir.irb.hr/id/eprint/6840
DOI:	10.1177/20587392211065613

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