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Bowel adhesion and therapy with the stable gastric pentadecapeptide BPC 157, L-NAME and L-arginine in rats

Berkopić Cesar, Lidija; Gojković, Slaven; Krezić, Ivan; Malekinušić, Dominik; Žižek, Helena; Batelja Vuletić, Lovorka; Petrović, Andreja; Horvat Pavlov, Katarina; Drmić, Domagoj; Kokot, Antonio; Vlainić, Josipa; Seiwerth, Sven; Sikirić, Predrag (2020) Bowel adhesion and therapy with the stable gastric pentadecapeptide BPC 157, L-NAME and L-arginine in rats. World Journal of Gastrointestinal Pharmacology and Therapeutics, 11 (5). pp. 93-109. ISSN 2150-5349

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BACKGROUND After parietal peritoneum excision with an underlying superficial layer of muscle tissue in rats, there is failed vasculature, and finally, increased adhesion formation. We hypothesized that unlike nitric oxide (NO)-agents, L-NAME and/or L- arginine, the application of the stable gastric pentadecapeptide BPC 157 with its most recent vascular effects (“vascular recruitment”) means attenuated bowel adhesion formation and NO- and malondialdehyde (MDA)-tissue values. AIM To focused on the bowel adhesion and the therapy with the BPC 157, its most and application of NO- agents. METHODS Along with defect creation, medication was (1) during surgery, once, at 1 min after defect creation as an abdominal bath (1 mL/rat), BPC 157 (10 µg/kg, 10 ng/kg, 1 mL/rat), an equivolume of saline, L-NAME (5 mg/kg), L-arginine (200 mg/kg) alone and/or combined. Alternatively, medication was (2) intraperitoneally once daily, first application at 30 min after surgery, last application 24 h before assessment at d 7 or d 14. As a postponed therapy to pre-existing adhesion (3), BPC 157 (10 µg/kg, 10 ng/kg intraperitoneally, 1 mL/rat) was given once daily since d 7. RESULTS The recovery effect of the BPC 157 regimens goes with the presence of abundant vascular vessels in and near the defect, which occurs rapidly. Lastly, also applied as post-treatment, BPC 157 creates attenuated adhesions, minimal or no adhesion. Contrarily, NO-agents have diverse initial and final effects: The initial weakening of blood vessel disappearance and finally, severe worsening of adhesions (L-NAME) vs the initial weakening of blood vessel disappearance and finally, attenuation of adhesions formation (L-arginine), which counteract each other response given together. Importantly, BPC 157 maintains its beneficial effect also when given with NO-agents (L-NAME + BC 157 ; L-arginine + BPC 157 ; L-NAME + L-arginine + BPC 157). Finally, with respect to the increased NO- and MDA- values-adhesion tissue formation relation, unlike diverse effect of the NO-agents, the BPC 157 application effect regularly combines decrease on the increased NO- and MDA- values and the beneficial outcome (less adhesion formation). CONCLUSION BPC 157 therapy can be suited for the realization of the peritoneal defect healing with minimal or no adhesion formation. Berkopic Cesar L, Gojkovic S, Krezic I, Malekinusic D, Zizek H, Batelja Vuletic L, Petrovic A, Horvat Pavlov K, Drmic D, Kokot A, Vlainic J, Seiwerth S, Sikiric P. Bowel adhesion and therapy with the stable gastric pentadecapeptide BPC 157, L-NAME and L-arginine in rats. World J Gastrointest Pharmacol Ther 2020 ; 11(5): 93-109 [PMID: 33251034 DOI: 10.4292/wjgpt.v11.i5.93]

Item Type: Article
Uncontrolled Keywords: Abdominal wall defect ; Adhesions ; BPC 157 ; Vascular recruitment ; Nitric oxide-agents ; Rats
Subjects: INTERDISCIPLINARY AREAS OF KNOWLEDGE > Biotechnology in Biomedicine (natural science, biomedicine and healthcare, bioethics area
Divisions: Division of Molecular Medicine
Depositing User: Josipa Vlainić
Date Deposited: 15 Nov 2021 11:01
DOI: 10.4292/wjgpt.v11.i5.93

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