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Effect of low dose gamma irradiation on number and ratio of some peripheral blood cells in hatched chicks

Pejaković Hlede, Jadranka; Vince, Silvijo; Žura Žaja, Ivona; Majer, Marija; Vilić, Marinko (2021) Effect of low dose gamma irradiation on number and ratio of some peripheral blood cells in hatched chicks. Veterinaria, 70 (1). pp. 27-36. ISSN 0372-6827

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Abstract

The research was carried out to investigate the hematological response to low-dose gamma radiation in chickens hatched from irradiated eggs. The experimental group was irradiated one hour before incubation with a cobalt-60 (60Co) panoramic irradiator, and the control group were sham irradiated. Blood samples for the red blood cell (RBC) and white blood cell (WBC) counts and differential blood counts were taken on the 1st, 3rd, 5th, 7th and 10th day of life. Blood cell counting was performed using the Natt and Herrick method. Leukocytes were differentiated using microscopic examination of stained blood smears and a heterophil:lymphocyte (H:L) ratio was calculated. Our results demonstrate a reduced RBC and WBC indicating negative effects of low-dose radiation on the blood cell counts in chicks during the first week after hatching. A significant decrease in the number of red blood cells on the 5th and 7th day and in the number of white blood cells on the 1st and 3rd day was obtained. Moreover, the number of lymphocytes in one-day-old chicken blood increased, while the number of heterophils decreased in response to radiation, suggesting a better stress response in the exposed group. Further research is necessary to investigate the value of the H:L ratio as a diagnostic indicator for radiation stress response and to evaluate cells and tissue reactions in specific time frames and across different taxonomic groups challenged by the low radiation dose. The extent of embryonic oxidative damage and recovery mechanisms should be also further investigated.

Item Type: Article
Uncontrolled Keywords: Heterophil ; lymphocyte ; erythrocyte ; chicken embryo ; ionizing radiation
Subjects: NATURAL SCIENCES > Interdisciplinary Natural Sciences
Divisions: Division of Materials Chemistry
Depositing User: Marija Majer
Date Deposited: 29 Jun 2021 09:52
Last Modified: 29 Jun 2021 09:52
URI: http://fulir.irb.hr/id/eprint/6466
DOI: 10.51607/22331360.2021.70.1.27

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