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In situ abundance and carbon fixation activity of distinct anoxygenic phototrophs in the stratified seawater lake Rogoznica

(CSF-BABAS Collaboration) Pjevac, Petra; Dyksma, Stefan; Goldhammer, Tobias; Mujakić, Izabela; Koblížek, Michal; Mussmann, Marc; Amann, Rudolf; Orlić, Sandi (2019) In situ abundance and carbon fixation activity of distinct anoxygenic phototrophs in the stratified seawater lake Rogoznica. Environmental microbiology, 21 (10). pp. 3896-3908. ISSN 1462-2912

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Abstract

Sulfide‐driven anoxygenic photosynthesis is an ancient microbial metabolism that contributes significantly to inorganic carbon fixation in stratified, sulfidic water bodies. Methods commonly applied to quantify inorganic carbon fixation by anoxygenic phototrophs, however, cannot resolve the contributions of distinct microbial populations to the overall process. We implemented a straightforward workflow, consisting of radioisotope labeling and flow cytometric cell sorting based on the distinct autofluorescence of bacterial photo pigments, to discriminate and quantify contributions of co‐occurring anoxygenic phototrophic populations to in situ inorganic carbon fixation in environmental samples. This allowed us to assign 89.3 ±7.6% of daytime inorganic carbon fixation by anoxygenic phototrophs in Lake Rogoznica (Croatia) to an abundant chemocline‐ dwelling population of green sulfur bacteria (dominated by Chlorobium phaeobacteroides), whereas the co‐occurring purple sulfur bacteria (Halochromatium sp.) contributed only 1.8 ±1.4%. Furthermore, we obtained two metagenome assembled genomes of green sulfur bacteria and one of a purple sulfur bacterium which provides the first genomic insights into the genus Halochromatium, confirming its high metabolic flexibility and physiological potential for mixo‐ and heterotrophic growth.

Item Type: Article
Uncontrolled Keywords: green sulfur bacteria ; purple sulfur bacteria ; carbon fixation ; flow cytometry ; 14C
Subjects: NATURAL SCIENCES > Biology
NATURAL SCIENCES > Biology > Microbiology
NATURAL SCIENCES > Interdisciplinary Natural Sciences > Environmental Science
Divisions: Division of Materials Chemistry
Projects:
Project titleProject leaderProject codeProject type
Bioraznolikost i biotehnološka karakterizacija bakterija Jadranskog mora-UNSPECIFIED02.05/38HRZZ
Depositing User: Sandi Orlić
Date Deposited: 07 Dec 2020 10:46
Last Modified: 07 Dec 2020 10:46
URI: http://fulir.irb.hr/id/eprint/6033
DOI: 10.1111/1462-2920.14739

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