Sadžak, Anja; Mravljak, Janez; Maltar- Strmečki, Nadica; Arsov, Zoran; Baranović, Goran; Erceg, Ina; Kriechbaum, Manfred; Strasser, Vida; Přibyl, Jan; Šegota, Suzana
(2020)
The Structural Integrity of the Model Lipid
Membrane during Induced Lipid Peroxidation: The
Role of Flavonols in the Inhibition of Lipid
Peroxidation.
Antioxidants, 9
(5).
pp. 430-460.
ISSN 2076-3921
Abstract
The structural integrity, elasticity, and fluidity of lipid membranes are critical for cellular activities such as communication between cells, exocytosis, and endocytosis. Unsaturated lipids, the main components of biological membranes, are particularly susceptible to the oxidative attack of reactive oxygen species. The peroxidation of unsaturated lipids, in our case 1, 2-dioleoyl-sn-glycero-3- phosphocholine (DOPC), induces the structural reorganization of the membrane. We have employed a multi-technique approach to analyze typical properties of lipid bilayers, i.e., roughness, thickness, elasticity, and fluidity. We compared the alteration of the membrane properties upon initiated lipid peroxidation and examined the ability of flavonols, namely quercetin (QUE), myricetin (MCE), and myricitrin (MCI) at different molar fractions, to inhibit this change. Using Mass Spectrometry (MS) and Fourier Transform Infrared Spectroscopy (FTIR), we identified various carbonyl products and examined the extent of the reaction. From Atomic Force Microscopy (AFM), Force Spectroscopy (FS), Small Angle X- Ray Scattering (SAXS), and Electron Paramagnetic Resonance (EPR) experiments, we concluded that the membranes with inserted flavonols exhibit resistance against the structural changes induced by the oxidative attack, which is a finding with multiple biological implications. Our approach reveals the interplay between the flavonol molecular structure and the crucial membrane properties under oxidative attack and provides insight into the pathophysiology of cellular oxidative injury.
| Item Type: |
Article
|
| Uncontrolled Keywords: |
bilayer thickness ; elasticity ; flavonols ; fluidity ; lipid peroxidation ; myricetin ; myricitrin ; quercetin |
| Subjects: |
NATURAL SCIENCES > Physics |
| Divisions: |
Division of Physical Chemistry |
| Projects: |
| Project title | Project leader | Project code | Project type |
|---|
| Zaštitni mehanizmi i učinci flavonoida ugrađenih u nanonosače u modelnim membranama i neuronima-NanoFlavNeuroProtect | Suzana Šegota | IP-2016-06-8415 | HRZZ |
|
| Depositing User: |
Suzana Šegota
|
| Date Deposited: |
27 Nov 2020 13:59 |
| URI: |
http://fulir.irb.hr/id/eprint/6025 |
| DOI: |
10.3390/antiox9050430 |
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