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Study of the diacylglycerol composition in the liver and serum of mice with prediabetes and diabetes using MeV TOF-SIMS

Popović Hadžija, Marijana; Siketić, Zdravko; Hadžija, Mirko; Barac, Marko; Bogdanović Radović, Ivančica (2020) Study of the diacylglycerol composition in the liver and serum of mice with prediabetes and diabetes using MeV TOF-SIMS. Diabetes Research and Clinical Practice, 159 . ISSN 0168-8227

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Aims: Hepatic insulin resistance, induced by fat, occurs before peripheral resistance and leads to prediabetes and diabetes. If insulin resistance is detected earlier, lifestyle changes could prevent or delay disease development. Therefore, we analysed lipids in the liver and serum of prediabetic and diabetic mice by MeV TOF-SIMS with a focus on diacylglycerols (DAGs) as the best predictor of (liver) resistance. Methods: Glucose impairment was spontaneously developed or induced by HFD in NOD/LtJ mice, and prediabetic and diabetic mice were selected according to their glucose levels. MeV TOF-SIMS was applied to image the lipid distribution in the liver and to relatively quantify lipids related to insulin resistance in both the liver and serum. Results: The same lipids were detected in the liver and serum but with different intensities between mice. The intensity of DAGs and fatty acids was higher in the diabetic than that in the prediabetic liver. Imaging of liver tissue showed a more compact density of prediabetic (non-fatty) than diabetic liver with DAG remodelling in diabetes. DAGs, which are greatly increased in diabetic serum, were successfully detected and quantified already in prediabetes. Conclusion: MeV TOF-SIMS applied to the serum presents an excellent tool for in vivo monitoring of disease development over time.

Item Type: Article
Additional Information: The authors thank Ms. Marina Mars. for the excellent technical assistance. This work is supported through grant IP-2016-06-1698 "Development of the capillary microprobe MeV SIMS with the application on analysis of biological samples" funded by the Croatian Science Foundation (Hrvatska Zaklada za Znanost) and European Regional Development Fund for the "Centre of Excellence for Advanced Materials and Sensing Devices" (Grant No. KK.
Uncontrolled Keywords: Lipids ; Liver ; Serum ; Diabetes ; MeV TOF-SIMS
Subjects: NATURAL SCIENCES > Interdisciplinary Natural Sciences
Divisions: Division of Experimental Physics
Division of Molecular Medicine
Project titleProject leaderProject codeProject type
Razvoj kapilarne mikroprobe za MeV SIMS s primjenom na analizu bioloških materijala-BioCapSIMSIvančica Bogdanović RadovićIP-2016-06-1698HRZZ
Depositing User: Ivančica Bogdanović Radović
Date Deposited: 31 Mar 2020 08:20
DOI: 10.1016/j.diabres.2019.107986

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