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Inducible ASABF-Type Antimicrobial Peptide from the Sponge Suberites domuncula: Microbicidal and Hemolytic Activity in Vitro and Toxic Effect on Molluscs in Vivo

Wiens, Matthias; Schroder, Heinz C.; Korzhev, Michael; Wang, Xiao-Hong; Batel, Renato; Muller, Werner E.G. (2011) Inducible ASABF-Type Antimicrobial Peptide from the Sponge Suberites domuncula: Microbicidal and Hemolytic Activity in Vitro and Toxic Effect on Molluscs in Vivo. Marine Drugs, 9 (10). pp. 1969-1994. ISSN 1660-3397

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Abstract

Since sponges, as typical filter-feeders, are exposed to a high load of attacking prokaryotic and eukaryotic organisms, they are armed with a wide arsenal of antimicrobial/cytostatic low-molecular-weight, non-proteinaceous bioactive compounds. Here we present the first sponge agent belonging to the group of ASABF-type antimicrobial peptides. The ASABF gene was identified and cloned from the demospongeSuberites domuncula. The mature peptide, with a length of 64 aa residues has a predicted pI of 9.24, and comprises the characteristic CSαβ structural motif. Consequently, the S. domuncula ASABF shares high similarity with the nematode ASABFs ; it is distantly related to the defensins. The recombinant peptide was found to display besides microbicidal activity, anti-fungal activity. In addition, the peptide lyses human erythrocytes. The expression ofASABF is upregulated after exposure to the apoptosis-inducing agent 2, 2'-dipyridyl. During the process of apoptosis of surface tissue of S. domuncula, grazing gastropods (Bittium sp.) are attracted by quinolinic acid which is synthesized through the kynurenine pathway by the enzyme 3-hydroxyanthranilate 3, 4-dioxygenase (HAD). Finally, the gastropods are repelled from the sponge tissue by the ASABF. It is shown that the effector peptide ASABF is sequentially expressed after the induction of the HAD gene and a caspase, as a central enzyme executing apoptosis.

Item Type: Article
Uncontrolled Keywords: sponges; Suberites domuncula; ASABF; antimicrobial peptides; apoptosis; Bittium sp.
Subjects: NATURAL SCIENCES > Geology
Divisions: Center for Marine Research
Projects:
Project titleProject leaderProject codeProject type
Učinak zagađenja na programirane biosinteze morskih beskralješnjakaRenato Batel098-0982705-2724MZOS
Depositing User: Renato Batel
Date Deposited: 10 Mar 2020 15:25
Last Modified: 10 Mar 2020 15:25
URI: http://fulir.irb.hr/id/eprint/5330
DOI: 10.3390/md9101969

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