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Isolation and Characterization of a Mn(II)-Oxidizing Bacillus Strain from the Demosponge Suberites domuncula

Wang, Xiaohong; Wiens, Matthias; Divekar, Mugdha; Grebenjuk, Vladislav A.; Schroeder, Heinz C.; Batel, Renato; Mueller, Werner E.G. (2011) Isolation and Characterization of a Mn(II)-Oxidizing Bacillus Strain from the Demosponge Suberites domuncula. Marine Drugs, 9 (1). pp. 1-28. ISSN 1660-3397

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Abstract

In this study we demonstrate that the demosponge Suberites domuncula harbors a Mn(II)-oxidizing bacterium, a Bacillus strain, termed BAC-SubDo-03. Our studies showed that Mn(II) stimulates bacterial growth and induces sporulation. Moreover, we show that these bacteria immobilize manganese on their cell surface. Comparison of the 16S rDNA sequence allowed the grouping of BAC-SubDo-03 to the Mn-precipitating bacteria. Analysis of the spore cell wall revealed that it contains an Mn(II)-oxidizing enzyme. Co-incubation studies of BAC-SubDo-03 with 100 mu M MnCl(2) and >1 mu M of CuCl(2) showed an increase in their Mn(II)-oxidizing capacity. In order to prove that a multicopper oxidase-like enzyme(s) (MCO) exists in the cell wall of the S. domuncula-associated BAC-SubDo-03 Bacillus strain, the gene encoding this enzyme was cloned (mnxG-SubDo-03). Sequence alignment of the deduced MCO protein (MnxG-SubDo-03) revealed that the sponge bacterium clusters together with known Mn(II)-oxidizing bacteria. The expression of the mnxG-SubDo-03 gene is under strong control of extracellular Mn(II). Based on these findings, we assume that BAC-SubDo-03 might serve as a Mn reserve in the sponge providing the animal with the capacity to detoxify Mn in the environment. Applying the in vitro primmorph cell culture system we could demonstrate that sponge cells, that were co-incubated with BAC-SubDo-03 in the presence of Mn(II), show an increased proliferation potential.

Item Type: Article
Uncontrolled Keywords: Suberites domuncula; Mn-precipitating bacteria; Mn(II)-oxidizing enzyme
Subjects: NATURAL SCIENCES > Geology
Divisions: Center for Marine Research
Projects:
Project titleProject leaderProject codeProject type
Učinak zagađenja na programirane biosinteze morskih beskralješnjakaRenato Batel098-0982705-2724MZOS
Depositing User: Renato Batel
Date Deposited: 10 Mar 2020 14:54
Last Modified: 10 Mar 2020 14:54
URI: http://fulir.irb.hr/id/eprint/5328
DOI: 10.3390/md9010001

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