hrvatski jezikClear Cookie - decide language by browser settings

Characterization of Nme5-Like Gene/Protein from the Red Alga Chondrus Crispus

Perina, Dragutin; Korolija, Marina; Mikoč, Andreja; Halasz, Mirn; Herak Bosnar, Maja; Ćetković, Helena (2020) Characterization of Nme5-Like Gene/Protein from the Red Alga Chondrus Crispus. Marine Drugs, 18 (1). ISSN 1660-3397

[img]
Preview
PDF - Published Version - article
Available under License Creative Commons Attribution.

Download (37MB) | Preview

Abstract

The Nme gene/protein family of nucleoside diphosphate kinases (NDPK) was originally named after its member Nm23-H1/Nme1, the first identified metastasis suppressor. Human Nme proteins are divided in two groups. They all possess nucleoside diphosphate kinase domain (NDK). Group I (Nme1-Nme4) display a single type NDK domain, whereas Group II (Nme5-Nme9) display a single or several different NDK domains, associated or not associated with extra- domains. Data strongly suggest that, unlike Group I, none of the members of Group II display measurable NDPK activity, although some of them autophosphorylate. The multimeric form is required for the NDPK activity. Group I proteins are known to multimerize, while there are no data on the multimerization of Group II proteins. The Group II ancestral type protein was shown to be conserved in several species from three eukaryotic supergroups. Here, we analysed the Nme protein from an early branching eukaryotic lineage, the red alga Chondrus crispus. We show that the ancestral type protein, unlike its human homologue, was fully functional multimeric NDPK with high affinity to various types of DNA and dispersed localization throughout the eukaryotic cell. Its overexpression inhibits both cell proliferation and the anchorage-independent growth of cells in soft agar but fails to deregulate cell apoptosis. We conclude that the ancestral gene has changed during eukaryotic evolution, possibly in correlation with the protein function.

Item Type: Article
Uncontrolled Keywords: Nme gene/protein family ; Nme5 ; red alga ; Chondrus crispus ; eukaryotic evolution ; Nme evolution
Subjects: NATURAL SCIENCES > Biology
Divisions: Division of Molecular Biology
Division of Molecular Medicine
Projects:
Project titleProject leaderProject codeProject type
Geni i genomi: struktura, funkcija i evolucija-Helena Ćetković098-0982913-2874MZOS
UNSPECIFIEDUNSPECIFIEDKK.01.1.1.01UNSPECIFIED
Depositing User: Sofija Konjević
Date Deposited: 15 Jan 2020 08:46
Last Modified: 15 Jan 2020 08:46
URI: http://fulir.irb.hr/id/eprint/5295
DOI: 10.3390/md18010013

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year