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Cell-type-specific modulation of hydrogen peroxide cytotoxicity and 4-hydroxynonenal binding to human cellular proteins in vitro by antioxidant Aloe vera extract

Cesar, Vera; Jozić, Iva; Begović, Lidija; Vuković, Tea; Mlinarić, Selma; Lepeduš, Hrvoje; Borović Šunjić, Suzana; Žarković, Neven (2018) Cell-type-specific modulation of hydrogen peroxide cytotoxicity and 4-hydroxynonenal binding to human cellular proteins in vitro by antioxidant Aloe vera extract. Antioxidants, 7 (10). ISSN 2076-3921

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Abstract

Although Aloe vera contains numerous bioactive components, the activity principles of widely used A. vera extracts are uncertain. Therefore, we analyzed the effects of genuine A. vera aqueous extract (AV) on human cells with respect to the effects of hydrogen peroxide (H2O2) and 4-hydroxynonenal (HNE). Fully developed A. vera leaves were harvested and analyzed for vitamin C, carotenoids, total soluble phenolic content, and antioxidant capacity. Furthermore, human cervical cancer (HeLa), human microvascular endothelial cells (HMEC), human keratinocytes (HaCat), and human osteosarcoma (HOS) cell cultures were treated with AV extract for one hour after treatment with H2O2 or HNE. The cell number and viability were determined using Trypan Blue, and endogenous reactive oxygen species (ROS) production was determined by fluorescence, while intracellular HNE–protein adducts were measured for the first time ever by genuine cell-based HNE–His ELISA. The AV extract expressed strong antioxidant capacities (1.1 mmol of Trolox eq/g fresh weight) and cell- type-specific influence on the cytotoxicity of H2O2, as well as on endogenous production of ROS and HNE–protein adducts induced by HNE treatment, while AV itself did not induce production of ROS or HNE–protein adducts at all. This study, for the first time, revealed the importance of HNE for the activity principles of AV. Since HMEC cells were the most sensitive to AV, the effects of AV on microvascular endothelia could be of particular importance for the activity principles of Aloe vera extracts.

Item Type: Article
Uncontrolled Keywords: Aloe vera ; plant extract ; antioxidants ; cell growth ; oxidative sterss ; reactive oxygen species (ROS) ; hydrogen peroxide ; lipid peroxidation ; 4-hydroxynonenal (HNE) ; cell-based ELISA ; HNE-protein adducts ; microvascular endothelium
Subjects: NATURAL SCIENCES > Biology
Divisions: Division of Molecular Medicine
Depositing User: Tea Vuković
Date Deposited: 19 Dec 2019 13:46
Last Modified: 19 Dec 2019 13:47
URI: http://fulir.irb.hr/id/eprint/5267
DOI: 10.3390/antiox7100125

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