Distribution of selected metals among cytosolic biomolecules in gills and liver of chub (Squalius cephalus (Linnaeus) and Squalius vardarensis Karaman; Actinopterygii, Cyprinidae) from metal contaminated natural waters

Krasnići, Nesrete (2019) Distribution of selected metals among cytosolic biomolecules in gills and liver of chub (Squalius cephalus (Linnaeus) and Squalius vardarensis Karaman; Actinopterygii, Cyprinidae) from metal contaminated natural waters. Doctoral thesis, Sveučilište u Zagrebu, Prirodoslovno-matematički fakultet.

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Abstract

The cytosolic distributions of several selected elements (Cd, Co, Cu, Fe, Mn, Mo, Pb, Se, and Zn) in the liver and gills of two freshwater bioindicators, European chub (Squalius cephalus) from the Sutla River in Croatia and Vardar chub (Squalius vardarensis) from three rivers of north-eastern Macedonia, were studied using size-exclusion high performance liquid chromatography (SEC-HPLC) and high resolution inductively coupled plasma mass spectrometry (HR ICP-MS). Distributions of metals/non-metal among the biomolecules of different molecular masses which are characteristic for low metal exposure in the river water were determined, as well as the changes in distribution which occur as a consequence of increased exposure to metals. The use of anion-exchange high performance liquid chromatography (AEC-HPLC) as the second step of separation and purification of cytosolic biomolecules, as well as the use of two mass spectrometry techniques (MALDI-TOF-MS and LC-MS/MS) have enabled the identification or exact determination of molecular masses of several cytosolic biomolecules that bind metals in the liver and gills of Vardar chub, such as the metallothionein isoforms that bind Cd, Cu and Zn, the hemoglobin subunits that bind Fe and heat stable biomolecules that bind Mo. Applied analytical approach has proven to be useful in the study of intracellular fate and behavior of metals in organs of fish, as well as in the process of discovery of potential new biomarkers of metal exposure and effects.

Item Type:	Thesis (Doctoral thesis)
Uncontrolled Keywords:	European chub; Vardar chub; metals; cytosolic biomolecules; chromatography; mass spectrometry
Subjects:	NATURAL SCIENCES > Chemistry > Analytic Chemistry NATURAL SCIENCES > Biology
Divisions:	Division for Marine and Enviromental Research
Depositing User:	Nesrete Krasnići
Date Deposited:	09 Dec 2019 13:35
URI:	http://fulir.irb.hr/id/eprint/5243

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