hrvatski jezikClear Cookie - decide language by browser settings

Radio-sensitization of head and neck cancer cells by a combination of poly(I:C) and cisplatin through downregulation of survivin and c-IAP2

Matijević Glavan, Tanja (2018) Radio-sensitization of head and neck cancer cells by a combination of poly(I:C) and cisplatin through downregulation of survivin and c-IAP2. Cellular Oncology, 42 (1). pp. 29-40. ISSN 2211-3428

| Request a personal copy from author

Abstract

PURPOSE: Head and neck squamous cell carcinoma (HNSCC) is one of the most common cancers. Concurrent radio-chemotherapy is the standard of care for advanced tumors. However, there is a need for more efficient regimens with less side effects resulting from high doses. Therefore, we set out to explore the therapeutic potential of ternary combinations by bringing together irradiation, cis-platinum and a TLR3 agonist, poly(I:C), with the aim to reduce the dosage of each treatment. This approach is based on our previous work, which revealed a selective cytotoxic effect of TLR3 agonists against malignant cells when combined with other anti-neoplastic agents. METHODS: We explored the survival of HNSCC-derived cells (Detroit 562, FaDu, SQ20B and Cal27) using MTT and caspase 3/7 activation assays. The radio-sensitization effects of poly(I:C) and cisplatin were assessed using Western blotting, cell cycle progression, ROS formation and qRT-PCR assays. RESULTS: We found that the combination of poly(I:C) and cisplatin downregulated c-IAP2 and survivin expression, reduced cell survival, induced anti-apoptotic gene expression and apoptosis, increased ROS formation and induced G2/M cell cycle arrest in the HNSCC-derived cells tested. CONCLUSIONS: Our results indicate that a combined poly(I:C) and cisplatin treatment reduces the survival and induces the radio-sensitivity of HNSCC-derived cells, thus providing a rationale for the development of novel strategies for the treatment of head and neck cancer.

Item Type: Article
Uncontrolled Keywords: Cisplatin; Head and neck cancer; Poly(I:C); Radio-sensitization; TLR3; Therapy
Subjects: BIOMEDICINE AND HEALTHCARE > Basic Medical Sciences > Human Genetics, Genomics and Proteomics
Divisions: Division of Molecular Medicine
Depositing User: Tanja Matijević Glavan
Date Deposited: 21 Nov 2019 11:51
Last Modified: 21 Nov 2019 11:51
URI: http://fulir.irb.hr/id/eprint/5105
DOI: 10.1007/s13402-018-0403-7

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year