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Enhanced Protection of Biological Membranes during Lipid Peroxidation: Study of the Interactions between Flavonoid Loaded Mesoporous Silica Nanoparticles and Model Cell Membranes

Mandić, Lucija; Sadžak, Anja; Strasser, Vida; Baranović, Goran; Domazet Jurašin, Darija; Dutour Sikirić, Maja; Šegota, Suzana (2019) Enhanced Protection of Biological Membranes during Lipid Peroxidation: Study of the Interactions between Flavonoid Loaded Mesoporous Silica Nanoparticles and Model Cell Membranes. International Journal of Molecular Sciences, 20 . pp. 2709-2731. ISSN 1661-6596

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Abstract

Flavonoids, polyphenols with anti-oxidative activity have high potential as novel therapeutics for neurodegenerative disease, but their applicability is rendered by their poor water solubility and chemical instability under physiological conditions. In this study, this is overcome by delivering flavonoids to model cell membranes (unsaturated DOPC) using prepared and characterized biodegradable mesoporous silica nanoparticles, MSNs. Quercetin, myricetin and myricitrin have been investigated in order to determine the relationship between flavonoid structure and protective activity towards oxidative stress, i.e., lipid peroxidation induced by the addition of hydrogen peroxide and/or Cu2+ ions. Among investigated flavonoids, quercetin showed the most enhanced and prolonged protective anti-oxidative activity. The nanomechanical (Young modulus) measurement of the MSNs treated DOPC membranes during lipid peroxidation confirmed attenuated membrane damage. By applying a combination of experimental techniques (atomic force microscopy—AFM, force spectroscopy, electrophoretic light scattering—ES and dynamic light scattering—DLS), this work generated detailed knowledge about the effects of flavonoid loaded MSNs on the elasticity of model membranes, especially under oxidative stress conditions. Results from this study will pave the way towards the development of innovative and improved markers for oxidative stress-associated neurological disorders. In addition, the obtained could be extended to designing effective delivery systems of other high potential bioactive molecules with an aim to improve human health in general.

Item Type: Article
Uncontrolled Keywords: lipid peroxidation ; membrane elasticity ; mesoporous silica nanoparticles ; myricetin ; myricitrin ; nanomechanics ; protective effects of flavonoids ; quercetin
Subjects: NATURAL SCIENCES > Interdisciplinary Natural Sciences
Divisions: Division of Organic Chemistry and Biochemistry
Division of Physical Chemistry
Projects:
Project titleProject leaderProject codeProject type
Zaštitni mehanizmi i učinci flavonoida ugrađenih u nanonosače u modelnim membranama i neuronima-NanoFlavNeuroProtectSuzana ŠegotaIP-2016-06-8415HRZZ
Depositing User: Suzana Šegota
Date Deposited: 16 Jul 2019 12:27
Last Modified: 16 Jul 2019 12:28
URI: http://fulir.irb.hr/id/eprint/4876
DOI: 10.3390/ijms20112709

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