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New Brush-Type Chiral Stationary Phases for Enantioseparation of Pharmaceutical Drugs

Knežević, Anamarija; Novak, Jurica; Vinković, Vladimir (2019) New Brush-Type Chiral Stationary Phases for Enantioseparation of Pharmaceutical Drugs. Molecules, 24 (4). ISSN 1420-3049

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Abstract

The importance of chirality in drug development is unquestionable, with chiral liquid chromatography (LC) being the most adequate technique for its analysis. Among the various types of chiral stationary phases (CSPs) for LC, brush-type CSPs provide the base for interaction analysis of CSPs and enantiomers, which provide valuable results that can be applied to interaction studies of other CSP types. In order to analyze the influence of aromatic interactions in chiral recognition, we designed a set of ten new brush-type CSPs based on (S)-N-(1-aryl-propyl)-3, 5-dinitrobenzamides which differ in the aromatic unit directly linked to the chiral center. Thirty diverse racemates, including several nonsteroidal anti-inflammatory drugs and 3-hydroxybenzodiazepine drugs, were used to evaluate the prepared CSPs. Chromatographic analysis showed that the three new CSPs separate enantiomers of a wide range of compounds and their chromatographic behavior is comparable to the most versatile brush-type CSP—Whelk-O1. The critical role of the nonbonding interactions in positioning of the analyte (naproxen) in the cleft of CSP-6, as well as the analysis of interactions that make enantioseparation possible, were elucidated using computational methods. Furthermore, the influence of acetic acid as a mobile phase additive, on this enantiorecognition process was corroborated by calculations.

Item Type: Article
Uncontrolled Keywords: chiral chromatography ; chiral recognition ; intermolecular interactions ; chiral drugs ; Whelk-O1 column ; mobile phase additives
Subjects: NATURAL SCIENCES > Chemistry
Divisions: Division of Organic Chemistry and Biochemistry
Division of Physical Chemistry
Projects:
Project titleProject leaderProject codeProject type
Svjetlo na molekulama: istraživanje spregnute elektronske i nuklearne dinamike-LightMolNađa DošlićIP-2016-06-1142HRZZ
Depositing User: Anamarija Knežević
Date Deposited: 11 Jun 2019 10:04
Last Modified: 11 Jun 2019 10:04
URI: http://fulir.irb.hr/id/eprint/4538
DOI: 10.3390/molecules24040823

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