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Expression of small leucine-rich extracellular matrix proteoglycans biglycan and lumican reveals oral lichen planus malignant potential

Lončar-Brzak, Božana; Klobučar, Marko; Veliki-Dalić, Irena; Sabol, Ivan; Kraljević Pavelić, Sandra; Krušlin, Božo; Mravak-Stipetić, Marinka (2017) Expression of small leucine-rich extracellular matrix proteoglycans biglycan and lumican reveals oral lichen planus malignant potential. Clinical Oral Investigations . ISSN 1432-6981

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Abstract

Objectives The aim of this study was to examine molecular alterations on the protein level in lesions of oral lichen planus (OLP), oral squamous cell carcinoma (OSCC) and healthy mucosa. Materials and methods Global protein profiling methods based on liquid chromatography coupled to mass spectrometry (LC-MS) were used, with a special emphasis on evaluation of deregulated extracellular matrix molecules expression, as well as on analyses of IG2F and IGFR2 expression in healthy mucosa, OLP and OSCC tissues by comparative semi-quantitative immunohistochemistry. Results Mass spectrometry-based proteomics profiling of healthy mucosa, OLP and OSCC tissues (and accompanied histologically unaltered tissues, respectively) identified 55 extracellular matrix proteins. Twenty among identified proteins were common to all groups of samples. Expression of small leucine-rich extracellular matrix proteoglycans lumican and biglycan was found both in OSCC and OLP and they were validated by Western blot analysis as putative biomarkers. A significant increase (p < 0.05) of biglycan expression in OLP-AT group was determined in comparison with OLP-T group, while lumican showed significant up-regulation (p < 0.05) in OLP-T and OSCC-T groups vs. adjacent and control tissue groups. Biglycan expression was only determined in OSCC-AT group. Immunohistochemical analysis of IGF2 and IG2FR expression revealed no significant difference among groups of samples. Conclusion/clinical relevance Biglycan and lumican were identified as important pathogenesis biomarkers of OLP that point to its malignant potential.

Item Type: Article
Uncontrolled Keywords: oral lichen planus ; proteomics ; biglycan ; lumican ; IGF2 ; IGF2R
Subjects: BIOMEDICINE AND HEALTHCARE > Basic Medical Sciences
BIOMEDICINE AND HEALTHCARE > Dental Medicine
Divisions: Division of Molecular Medicine
Projects:
Project titleProject leaderProject codeProject type
Molekularni mehanizmi nastanka prekanceroznih i kanceroznih lezija usne šupljine-Marinka Mravak-Stipetić065-0982464-2532MZOS
Depositing User: Ivan Sabol
Date Deposited: 17 Jan 2018 13:42
Last Modified: 17 Jan 2018 13:42
URI: http://fulir.irb.hr/id/eprint/3808
DOI: 10.1007/s00784-017-2190-3

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