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Detection and diversity of aeromonads from treated wastewater and fish inhabiting effluent and downstream waters

Topić Popović, Natalija; Kazazić, Snježana; Strunjak-Perović, Ivančica; Barišić, Josip; Sauerborn Klobučar, Roberta; Kepec, Slavko; Čož- Rakovac, Rozelinda (2015) Detection and diversity of aeromonads from treated wastewater and fish inhabiting effluent and downstream waters. Ecotoxicology and Environmental Safety, 120 . pp. 235-242. ISSN 0147-6513

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Abstract

A two-season investigation of the wastewater treatment plant (WWTP) effluent, of related waters, sludge and fish across a wide area and 11 stations, with emphasis on Aeromonas spp. was conducted. Aeromonas veronii was the prevailing aeromonad isolated by MALDI TOF MS in the summer period. A rise of A. hydrophila was observed in summer in raw sewage, treated wastewater and effluent-carrying canal. The ratio of aeromonad species retrieved from fish tissues did not correspond with the water and sludge findings, as in spring in the effluent-carrying canal fish carried A. salmonicida ssp. salmonicida and A. bestiarum, while in summer mainly A. veronii and A. johnsonii were isolated from fish tissues in the same location. No correlation was established between fecal coliforms/enterococci and aeromonad occurrence. All retrieved Aeromonas species demonstrated a distinct spectral pattern, with peaks showing unique mass distribution ranging from 4, 000 to 10, 000 Da. Hierarchical clustering separated aeromonads of all isolated species and clustered closely related strains together. Resistance was determined towards amoxicillin, and frequently towards sulfamethoxazole and erythromycin. In summer, a high proportion of water and sludge Aeromonas species demonstrated multiple resistance patterns towards five or more antimicrobials. The quinolone resistance of water aeromonads was mostly related to A. veronii. There are potential health concerns regarding aeromonad exposure amongst recreational fishermen who come into contact with fish inhabiting waters downstream from the WWTP, and WWTP workers who are occupationally exposed to wastewaters and their aerosols.

Item Type: Article
Additional Information: This work was supported by the Croatian MSES Grant no. 098-1782739-249. The authors declare no conflict of interest.
Uncontrolled Keywords: WWTP; Aeromonas; MALDI TOF MS; API 20E; wastewater
Subjects: NATURAL SCIENCES > Biology
Divisions: Division of Materials Chemistry
Division of Physical Chemistry
Projects:
Project titleProject leaderProject codeProject type
Croatian MSES GrantUNSPECIFIED098-1782739-249UNSPECIFIED
Depositing User: Natalija Topić Popović
Date Deposited: 07 Dec 2017 12:09
Last Modified: 07 Dec 2017 12:09
URI: http://fulir.irb.hr/id/eprint/3756
DOI: 10.1016/j.ecoenv.2015.06.011

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