Differentiation of environmental aquatic bacterial isolates by MALDI-TOF MS

Topić Popović, Natalija; Kazazić, Snježana; Strunjak-Perović, Ivančica; Čož-Rakovac, Rozelinda (2017) Differentiation of environmental aquatic bacterial isolates by MALDI-TOF MS. Environmental Research, 152 . pp. 7-16. ISSN 0013-9351

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Abstract

Identification of bacteria in aquatic and environmental applications, for monitoring purposes and research, for health assessments and therapy considerations of farmed and free- living aquatic organisms, still relies on conventional phenotypic and biochemical protocols. Although molecular techniques based on DNA amplification and sequencing are finding ways into diagnostic laboratories, they are time-consuming, costly and difficult in the case of multiplex assays. Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) is a rapid and accurate proteomic method reliable for identification of unknown bacteria to the genus and species level. Upon extension of databases, it will certainly find its position in environmental sciences. The paper presents an overview of the principle of the method, its effectiveness in comparison with conventional and molecular identification procedures, and applicability on environmental and aquatic isolates, discussing its advantages and shortcomings, as well as possible future implementations.

Item Type:

Article

Additional Information:

This work was supported by the Ministry of Science, Education and Sports of the Republic Croatia [Grant no. 098-1782739-2749, 098-0982915-2945).

Uncontrolled Keywords:

MALDI-TOF MS ; environmental bacteria ; fish ; crayfish

Subjects:

BIOTECHNICAL SCIENCES > Biotechnology

Divisions:

Division of Materials Chemistry
Division of Physical Chemistry

Projects:

Project title	Project leader	Project code	Project type
Substanična biokemijska i filogenetska raznolikost tkiva riba, rakova i školjaka-	Rozelinda Čož-Rakovac	098-1782739-2749	MZOS
Spektroskopija, kemijska svojstva i reakcije biološki aktivnih molekula-	Branka Kovač	098-0982915-2945	MZOS

Depositing User:

Natalija Topić Popović

Date Deposited:

07 Dec 2017 10:19

URI:

http://fulir.irb.hr/id/eprint/3744

DOI:

10.1016/j.envres.2016.09.020