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First evidence of the P-glycoprotein gene expression and multixenobiotic resistance modulation in earthworm

Bošnjak, Ivana; Bielen, Ana; Babić, Sanja; Šver, Lidija; Topić Popović, Natalija; Strunjak-Perović, Ivančica; Čož-Rakovac, Rozelinda; Klobučar, Roberta Sauerborn (2014) First evidence of the P-glycoprotein gene expression and multixenobiotic resistance modulation in earthworm. Archives of Industrial Hygiene and Toxicology, 65 (1). pp. 67-74. ISSN 0004-1254

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Abstract

Multixenobiotic resistance (MXR) is an important mechanism of cellular efflux mediated by ATP binding cassette (ABC) transporters that bind and actively remove toxic substrates from the cell. This study was the first to identify ABC transporter P-glycoprotein (P-gp/ABCB1) as a representative of the MXR phenotype in earthworm (Eisenia fetida). The identified partial cDNA sequence of ABCB1 overlapped with ABCB1 homologues of other organisms from 58.5 % to 72.5 %. We also studied the effect of five modulators (verapamil, cyclosporine A, MK571, probenecid, and orthovanadate) on the earthworm’s MXR activity by measuring the accumulation of model substrates rhodamine B and rhodamine 123 in whole body tissue of the adult earthworm. MK571, orthovanadate, and verapamil significantly inhibited MXR activity, and rhodamine 123 turned out to better reflect MXR activity in that species than rhodamine B. Our results show that E. fetida can serve well as a test organism for environmental pollutants that inhibit MXR activity.

Item Type: Article
Additional Information: This work was supported by the Ministry of Science, Education and Sports of the Republic of Croatia, Project No 058-0582261-2246 and 098-1782739-2749. We thank Dr Sasa Likic (Faculty of Science, Zagreb, Croatia) for technical help with the FLUOstar OPTIMA plate reader.
Uncontrolled Keywords: ABC transport proteins; cellular detoxification; cyclosporine A; Eisenia fetida; MK571; MXR; orthovanadate; probenecid; soil organism; verapamil
Subjects: NATURAL SCIENCES > Biology > Ecology
NATURAL SCIENCES > Interdisciplinary Natural Sciences > Environmental Science
Divisions: Division of Materials Chemistry
Projects:
Project titleProject leaderProject codeProject type
Utjecaj mutagena i antimutagena na molekularne procese u stanici-Reno Hrašćan058-0582261-2246MZOS
Substanična biokemijska i filogenetska raznolikost tkiva riba, rakova i školjaka-Rozelinda Čož-Rakovac098-1782739-2749MZOS
Depositing User: Sanja Babić
Date Deposited: 07 Dec 2017 09:57
Last Modified: 07 Dec 2017 09:57
URI: http://fulir.irb.hr/id/eprint/3731
DOI: 10.2478/10004-1254-65-2014-2421

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