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The effect of 17β-estradiol on the expression of dipeptidyl peptidase III and heme oxygenase 1 in liver of CBA/H mice

Mačak Šafranko, Željka; Sobočanec, Sandra; Šarić, Ana; Jajčanin-Jozić, Nina; Krsnik, Željka; Aralica, Gorana; Balog, Tihomir; Abramić, Marija (2015) The effect of 17β-estradiol on the expression of dipeptidyl peptidase III and heme oxygenase 1 in liver of CBA/H mice. Journal of Endocrinological Investigation, 38 (4). pp. 471-479. ISSN 0391-4097

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BACKGROUND: 17β-estradiol (E₂) has well-established cardioprotective, antioxidant and neuroprotective role, and exerts a vast range of biological effects in both sexes. Dipeptidyl peptidase III (DPP III) is protease involved as activator in Keap1-Nrf2 signalling pathway, which is important in cellular defense to oxidative and electrophilic stress. It is generally accepted that oxidative stress is crucial in promoting liver diseases. OBJECTIVE: To examine the effect of E₂ on the expression of DPP III and haeme oxygenase 1 (HO-1) in liver of adult CBA/H mice of both sexes. METHODS: Gene and protein expressions of studied enzymes were determined by quantitative real-time PCR and Western blot analysis. Immunohistochemistry was performed to analyse the localization of both proteins in different liver cell types. RESULTS: Ovariectomy diminished expression of DPP III and HO-1 proteins. E₂ administration abolished this effect, and even increased these proteins above the control. A significant enhancement in DPP III protein was found in E₂-treated males, as well. A decrease in the expression of HO-1, but not of the DPP III gene, was detected in the liver of ovariectomized females. HO-1 protein was found localized in the pericentral areas of hepatic lobules (Kupffer cells and hepatocytes), whilst DPP III showed a uniform distribution within hepatic tissue. CONCLUSIONS: We demonstrate for the first time that E₂ influences the protein level of DPP III in vivo, and confirm earlier finding on HO-1 gene upregulation by 17β-estradiol. These results additionally confer new insights into complexity of protective action of E₂.

Item Type: Article
Additional Information: The authors would like to thank Iva Pesun Medimorec for her excellent technical contribution in performing surgical procedures of ovariectomy and Ana Jagust for her excellent technical assistance in performing immunohistochemical analyses. This research is funded by Croatian Ministry of Science, Education and Sports, Grant No. 098-0982464-1647, Grant No. 098-1191344-2938 and IBRO 2013 RHP.
Uncontrolled Keywords: antioxidant enzyme; gene and protein expression; immunohistochemistry; ovariectomy; sex-related; steroid hormone; zinc peptidase
BIOMEDICINE AND HEALTHCARE > Basic Medical Sciences > Human Physiology
Divisions: Division of Molecular Medicine
Project titleProject leaderProject codeProject type
Sustav citokroma P450 i pojava tumora u starenju i oksidacijskom stresu-Tihomir Balog098-0982464-1647MZOS
Molekularna enzimologija i proteinske interakcije hidrolaza-Marija Abramić098-1191344-2938MZOS
Depositing User: Sandra Sobočanec
Date Deposited: 06 Dec 2017 14:54
DOI: 10.1007/s40618-014-0217-z

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