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Non-toxic fluorescent phosphonium probes to detect mitochondrial potential

Šarić, Ana; Sobočanec, Sandra; Bouillaud, Frédéric; Sobočanec, Sandra; Mikecin, Ana-Matea; Mačak Šafranko, Željka; Delgeorgiev, Todor; Piantanida, Ivo; Balog, Tihomir; Petit, Patrice X (2017) Non-toxic fluorescent phosphonium probes to detect mitochondrial potential. Methods and Applications in Fluorescence, 5 (1). ISSN 2050-6120

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Abstract

We evaluated our phosphonium-based fluorescent probes for selective staining of mitochondria. Currently used probes for monitoring mitochondrial membrane potential show varying degrees of interference with cell metabolism, photo-induced damage and probe binding. Here presented probes are characterised by highly efficient cellular uptake and specific accumulation in mitochondria. Fluorescent detection of the probes was accomplished using flow cytometry and confocal microscopy imaging of yeast and mammalian cells. Toxicity analysis (impedimetry-xCELLigence for the cellular proliferation and Seahorse technology for respiratory properties) confirms that these dyes exhibit no-toxicity on mitochondrial or cellular functioning even for long time incubation. The excellent chemical and photophysical stability of the dyes makes them promising leads toward improved fluorescent probes. Therefore, the probes described here offer to circumvent the problems associated with existing-probe's limitations.

Item Type: Article
Additional Information: This project has been funded by EU Marie Curie FP7PEOPLE-2011-COFUND program, NEWELPRO, European Commission Grant Agreement No. 291823; Ministry of Science, Education, Sport of Croatia Grant Numbers 098-0982464-13 and 098-0982914-2918; and InnoMol FP7-REGPOT-2012-2013-1 Grant Agreement Number 316289. Anita Krisko from MedILS is acknowledged for kindly providing us all S. cerevisiae cultures. Lucija Horvat is acknowledged for help in confocal microscopy.
Uncontrolled Keywords: mitochondria; membrane potential; flow cytometry; confocal microscopy; toxicity analysis; respiration; probe
Subjects: BIOMEDICINE AND HEALTHCARE
Divisions: Division of Molecular Medicine
Division of Organic Chemistry and Biochemistry
Projects:
Project titleProject leaderProject codeProject type
Sirtuin3 kao posrednik mitohondrijske funkcije u estrogen-ovisnoj otpornosti na oksidativni stres i prehranu s visokim udjelom masti-SuMERATihomir BalogIP-2014-09-4533HRZZ
Depositing User: Sandra Sobočanec
Date Deposited: 29 Apr 2020 08:17
Last Modified: 29 Apr 2020 10:57
URI: http://fulir.irb.hr/id/eprint/3720
DOI: 10.1088/2050-6120/aa5e64

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