3-Dimensional reconstruction of fluorescent structures in tardigrades

Pfannkuchen, Martin; Brümer, Franz; Schill, Ralph (2007) 3-Dimensional reconstruction of fluorescent structures in tardigrades. Journal of limnology, 66 (1). pp. 158-163. ISSN 1129-5767

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Abstract

Tardigrades are microscopic animals, thus brightfield microscopy is a well established method for tardigrade observation. Modern techniques in functional genetics like fluorescence in situ hybridisation or fluorescently labelled expression markers demand high resolution fluorescence microscopy. Nevertheless tardigrades are still considered to be difficult objects for fluorescence techniques as they are covered by an opaque and diffracting cuticle. We show a modern technique of structured light illumination that enables us to acquire thin optical sections and consequently to reconstruct 3-dimensional structures in tardigrades with a high spatial resolution in all 3 dimensions. This technique is evaluated on taxonomically valuable internal as well as external structures of eutardigrades: the bucco-pharyngeal apparatus and the claws. The 3-dimensional reconstructions allow the measurement of distances in all 3 dimensions.

Item Type:	Article
Uncontrolled Keywords:	3-dimensional rendering ; ApoTome ; Fluorescence microscopy ; Measuring 3D structures ; Optical sectioning ; Structured illumination ; Tardigrada
Subjects:	NATURAL SCIENCES > Biology
Divisions:	Center for Marine Research
Depositing User:	Martin Andreas Pfannkuchen
Date Deposited:	09 Aug 2017 13:03
URI:	http://fulir.irb.hr/id/eprint/3526
DOI:	10.4081/jlimnol.2007.s1.158

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