Šarić, Ana; Sobočanec, Sandra; Mačak Šafranko, Željka; Popović Hadžija, Marijana; Bagarić, Robert; Farkaš, Vladimir; Švarc, Alfred; Marotti, Tatjana; Balog, Tihomir (2015) Diminished resistance to hyperoxia in brains of reproductively senescent female CBA/H mice. Medical Science Monitor Basic Research, 21 . pp. 191-199. ISSN 2325-4394
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Abstract
Background: We have explored sex differences in ability to maintain redox balance during acute oxidative stress in mice brain. We aimed to study if there were differences in oxidative/antioxidative status upon hyperoxia in brain of reproductively senescent CBA/H mice in order to elucidate some of the possible mechanisms of lifespan regulation. Material and Methods: The brains of 12 months old male and female CBA/H mice (n=9 per sex and treatment) subjected to 18h hyperoxia were evaluated for lipid peroxidation (LPO), antioxidative enzyme expression and activity - superoxide dismutase 1 and 2 (Sod-1, Sod-2), catalase (Cat), glutathione peroxidase 1 (Gpx- 1), heme-oxygenase 1 (Ho-1), nad NF-E2-related factor 2 (Nrf2), and for 2-deoxy-2-[18F] fluoro-D-glucose (18FDG) uptake. Results: No increase in LPO was observed after hyperoxia, regardless of sex. Expression of Nrf-2 showed significant downregulation in hyperoxia-treated males (p=0.001), and upregulation in hyperoxia-treated females (p=0.023). Also, in females hyperoxia upregulated Sod-1 (p=0.046), and Ho-1 (p=0.014) genes. SOD1 protein was upregulated in both sexes after hyperoxia (p=0.009 for males and p=0.011 for females). SOD2 protein was upregulated only in females (p=0.008) while CAT (p=0.026) and HO-1 (p=0.042) proteins were increased after hyperoxia only in males. Uptake of 18FDG was decreased after hyperoxia in the backbrain of females. Conclusion: We found that females at their reproductive senescence are more susceptible to hyperoxia, compared to males. We propose this model of hyperoxia as a useful tool to assess sex differences in adaptive response to acute stress conditions, which may be partially responsible for observed sex differences in longevity of CBA/H mice.
| Item Type: | Article | ||||||||||||||||
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| Uncontrolled Keywords: | cell aging; fluorodeoxyglucose F18; hyperoxia; mice; inbred CBA; neuroimaging | ||||||||||||||||
| Subjects: | BIOMEDICINE AND HEALTHCARE > Basic Medical Sciences | ||||||||||||||||
| Divisions: | Division of Experimental Physics Division of Molecular Medicine |
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| Depositing User: | Marijana Popović Hadžija | ||||||||||||||||
| Date Deposited: | 02 Feb 2017 13:23 | ||||||||||||||||
| URI: | http://fulir.irb.hr/id/eprint/3363 | ||||||||||||||||
| DOI: | 10.12659/MSMBR.895356 |
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