hrvatski jezikClear Cookie - decide language by browser settings

Modelling of the gas-phase phosphate group loss and rearrangement in phosphorylated peptides

Rožman, Marko (2011) Modelling of the gas-phase phosphate group loss and rearrangement in phosphorylated peptides. Journal of Mass Spectrometry, 46 (9). pp. 949-955. ISSN 1076-5174

[img]
Preview
PDF - Accepted Version - article
Available under License Creative Commons Attribution Non-commercial.

Download (450kB) | Preview

Abstract

The gas-phase dissociation of phosphorylated peptides was modelled using a combination of quantum mechanics and the Rice–Ramsperger–Kassel–Marcus theory. Potential energy surfaces and unimolecular reaction rates for several low-energy fragmentation and rearrangement pathways were estimated, and a general mechanism was proposed. The neutral loss of the phosphoric acid was mainly an outcome of the intramolecular nucleophilic substitution mechanism. The mechanism involves a nucleophilic attack of the phosphorylated amino acid N-terminal carbonyl oxygen on b-carbon, yielding a cyclic five-membered oxazoline product ion. Regardless of the proton mobility, the pathway was charge directed either by a mobile proton or by a positively charged side chain of some basic residue. Although the mechanistic aspects of the phosphate loss are not influenced by the proton mobility environment, it does affect ion abundances. Results suggest that under the mobile proton environment, the interplay between phosphoric acid neutral loss product ion and backbone cleavage fragments should occur. On the other hand, when proton mobility is limited, neutral loss product ion may predominate. The fragmentation dynamics of phosphoserine versus phosphothreonine containing peptides suggests that H3PO4 neutral loss from phosphothreonine containing peptides is less abundant than that from their phosphoserine containing analogs. During the low-energy CID of phosphorylated peptides in the millisecond time range, typical for ion trap instruments, a phosphate group rearrangement may happen, resulting in an interchange between the phosphorylated and the hydroxylated residues. Unimolecular dissociation rate constants imply the low abundance of such scrambled product ions.

Item Type: Article
Uncontrolled Keywords: molecular modelling; fragmentation mechanisms; collision induced dissociation; phosphorylation; reaction mechanisms
Subjects: NATURAL SCIENCES > Chemistry
Divisions: Division of Physical Chemistry
Projects:
Project titleProject leaderProject codeProject type
Spektroskopija, kemijska svojstva i reakcije biološki aktivnih molekula-Branka Kovač098-0982915-2945MZOS
Depositing User: Kristina Ciglar
Date Deposited: 20 Jan 2017 14:35
Last Modified: 20 Jan 2017 14:35
URI: http://fulir.irb.hr/id/eprint/3339
DOI: 10.1002/jms.1974

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year