Proton transfer reactions for improved peptide characterisation

Rožman, Marko; Schneider, Andrea; Gaskell, Simon J. (2011) Proton transfer reactions for improved peptide characterisation. Journal of Mass Spectrometry, 46 (6). pp. 529-534. ISSN 1076-5174

Preview

PDF - Accepted Version - article
Available under License Creative Commons Attribution Non-commercial.
Download (149kB) | Preview

Official URL: http://onlinelibrary.wiley.com/doi/10.1002/jms.192...

Abstract

The combination of deprotonation (via ion/molecule and ion/ion reactions) and low-energy collision-induced dissociation (CID) has been explored for the enhanced characterisation of tryptic peptides via access to different precursor charge states. This approach allows instant access to fragmentation properties of singly and doubly protonated precursors (arising from the availability of mobile protons) in a single experiment. Considering both charge states extended our base of structurally informative data (in comparison with considering just a single charge state) due to generation of additional sequence ions and by obtaining supplementary structural information derived from selective cleavages. Roughly 37% of combined data sets (CID spectra of doubly and singly charged precursor) showed a greater database identification confidence than each set alone. Moreover, comparison between a number of sequence ions of the singly charged precursor and the doubly charged precursor provided a mean of distinguishing the two classes of tryptic peptides (arginine or lysine containing).

Item Type:

Article

Additional Information:

"This is the peer reviewed version of the following article: Rožman, Marko; Schneider, Andrea; Gaskell, Simon J. (2011) Proton transfer reactions for improved peptide characterisation. Journal of Mass Spectrometry, 46 (6). pp. 529-534, which has been published in final form http://onlinelibrary.wiley.com/doi/10.1002/jms.1920/abstract . This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving."

Uncontrolled Keywords:

ion/molecule reaction; ion/ion reaction; collision induced dissociation; peptide identification; charge state

Subjects:

NATURAL SCIENCES > Chemistry

Divisions:

Division of Physical Chemistry

Projects: