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Diverse antibiotic resistance genes in dairy cow manure

Wichmann, Fabienne; Udiković-Kolić, Nikolina; Andrew, Sheila; Handelsman, Jo (2014) Diverse antibiotic resistance genes in dairy cow manure. mBio, 5 (2). e01017-13. ISSN 2150-7511

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Application of manure from antibiotic-treated animals to crops facilitates the dissemination of antibiotic resistance determinants into the environment. However, our knowledge of the identity, diversity, and patterns of distribution of these antibiotic resistance determinants remains limited. We used a new combination of methods to examine the resistome of dairy cow manure, a common soil amendment. Metagenomic libraries constructed with DNA extracted from manure were screened for resistance to beta-lactams, phenicols, aminoglycosides, and tetracyclines. Functional screening of fosmid and small-insert libraries identified 80 different antibiotic resistance genes whose deduced protein sequences were on average 50 to 60% identical to sequences deposited in GenBank. The resistance genes were frequently found in clusters and originated from a taxonomically diverse set of species, suggesting that some microorganisms in manure harbor multiple resistance genes. Furthermore, amid the great genetic diversity in manure, we discovered a novel clade of chloramphenicol acetyltransferases. Our study combined functional metagenomics with third-generation sequencing to significantly extend the roster of functional antibiotic resistance genes found in animal gut bacteria, providing a particularly broad resource for understanding the origins and dispersal of antibiotic resistance genes in agriculture and clinical settings.

Item Type: Article
Uncontrolled Keywords: antibiotic resistance; cow manure; functional metagenomics
Subjects: BIOTECHNICAL SCIENCES > Biotechnology
Divisions: Division for Marine and Enviromental Research
Depositing User: Nikolina Udiković Kolić
Date Deposited: 11 Jul 2016 10:31
DOI: 10.1128/mBio.01017-13

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