Dominant Epistasis Between Two Quantitative Trait Loci Governing Sporulation Efficiency in Yeast Saccharomyces cerevisiae

Bergman, Juraj; Mitrikeski, Petar; Brčić-Kostić, Krunoslav (2015) Dominant Epistasis Between Two Quantitative Trait Loci Governing Sporulation Efficiency in Yeast Saccharomyces cerevisiae. Food Technology and Biotechnology, 53 (4). pp. 367-378. ISSN 1330-9862

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Abstract

Sporulation efficiency in the yeast Saccharomyces cerevisiae is a well-established model for studying quantitative traits. A variety of genes and nucleotides causing different sporulation efficiencies in laboratory, as well as in wild strains, has already been extensively characterised (mainly by reciprocal hemizygosity analysis and nucleotide exchange methods). We applied a different strategy in order to analyze the variation in sporulation efficiency of laboratory yeast strains. Coupling classical quantitative genetic analysis with simulations of phenotypic distributions (a method we call phenotype modelling) enabled us to obtain a detailed picture of the quantitative trait loci (QTLs) relationships underlying the phenotypic variation of this trait. Using this approach, we were able to uncover a dominant epistatic inheritance of loci governing the phenotype. Moreover, a molecular analysis of known causative quantitative trait genes and nucleotides allowed for the detection of novel alleles, potentially responsible for the observed phenotypic variation. Based on the molecular data, we hypothesise that the observed dominant epistatic relationship could be caused by the interaction of multiple quantitative trait nucleotides distributed across a 60-kb QTL region located on chromosome XIV and the RME1 locus on chromosome VII. Furthermore, we propose a model of molecular pathways which possibly underlie the phenotypic variation of this trait.

Item Type:	Article
Uncontrolled Keywords:	budding yeast; sporulation inheritance; two-locus epistasis; QTL analysis; phenotype simulation
Subjects:	NATURAL SCIENCES > Mathematics
Divisions:	Division of Molecular Biology
Projects:	Project title Project leader Project code Project type The role of recombination in DNA repair and genome evolution (Uloga rekombinacije u popravku DNA i evoluciji genoma)- Krunoslav Brčić-Kostić 098-0982913-2867 MZOS
Depositing User:	Krunoslav Brčić-Kostić
Date Deposited:	06 Jul 2016 14:25
URI:	http://fulir.irb.hr/id/eprint/2897
DOI:	10.17113/ftb.53.04.15.3998

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