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Satellite DNA Modulates Gene Expression in the Beetle Tribolium castaneum after Heat Stress

Feliciello, Isidoro; Akrap, Ivana; Ugarković, Đurđica (2015) Satellite DNA Modulates Gene Expression in the Beetle Tribolium castaneum after Heat Stress. PLoS Genetics, 11 (8). e1005466/1-e1005466/18. ISSN 1553-7390

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Abstract

Non-coding repetitive DNAs have been proposed to perform a gene regulatory role, however for tandemly repeated satellite DNA no such role was defined until now. Here we provide the first evidence for a role of satellite DNA in the modulation of gene expression under specific environmental conditions. The major satellite DNA TCAST1 in the beetle Tribolium castaneum is preferentially located within pericentromeric heterochromatin but is also dispersed as single repeats or short arrays in the vicinity of protein-coding genes within euchromatin. Our results show enhanced suppression of activity of TCAST1-associated genes and slower recovery of their activity after long-term heat stress relative to the same genes without associated TCAST1 satellite DNA elements. The level of gene suppression is not influenced by the distance of TCAST1 elements from the associated genes up to 40 kb from the genes’ transcription start sites, but it does depend on the copy number of TCAST1 repeats within an element, being stronger for the higher number of copies. The enhanced gene suppression correlates with the enrichment of the repressive histone marks H3K9me2/3 at dispersed TCAST1 elements and their flanking regions as well as with increased expression of TCAST1 satellite DNA. The results reveal transient, RNAi based heterochromatin formation at dispersed TCAST1 repeats and their proximal regions as a mechanism responsible for enhanced silencing of TCAST1-associated genes. Differences in the pattern of distribution of TCAST1 elements contribute to gene expression diversity among T. castaneum strains after long-term heat stress and might have an impact on adaptation to different environmental conditions.

Item Type: Article
Uncontrolled Keywords: satelite DNA; thermal stresses; itrons
Subjects: NATURAL SCIENCES > Mathematics
Divisions: Division of Molecular Biology
Projects:
Project titleProject leaderProject codeProject type
Fast evolving portion of eukaryotic genome: evolutionary and functional studies (Evolucija i funkcija brzo evoluirajućeg dijela eukariotskog genoma)-Đurđica Ugarković098-0982913-2832MZOS
Depositing User: Đurđica Ugarković
Date Deposited: 05 Jul 2016 12:21
Last Modified: 01 Dec 2016 15:22
URI: http://fulir.irb.hr/id/eprint/2894
DOI: 10.1371/journal.pgen.1005466

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