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Rapid transient expression of human granulocyte-macrophage colony-stimulating factor in two industrial cultivars of tobacco (Nicotiana tabacum L.) by agroinfiltration

Vojta, Lea; Ljuma-Skupnjak, Lana; Budimir, Ankica; Vukičević, Slobodan; Fulgosi, Hrvoje (2015) Rapid transient expression of human granulocyte-macrophage colony-stimulating factor in two industrial cultivars of tobacco (Nicotiana tabacum L.) by agroinfiltration. Biotechnology Reports, 7 . pp. 81-86. ISSN 2215-017X

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Abstract

We report the production of hGM-CSF cytokine in leaves of industrial tobacco cultivars DH-17 and DH-27 by using Agrobacterium-mediated transient expression. We prove the concept that very high biomass industrial tobacco plants are suitable platforms for rapid, low cost production of foreign proteins. Successful transient expression of the GM-CSF was achieved in less than three months, opening the possibility for future applications of this approach in rapid response production of various proteins of non-plant origin in industrial tobacco.

Item Type: Article
Uncontrolled Keywords: tobacco; transient expression; GM-CSF; vacuum infiltration; heterologous expression
Subjects: BIOTECHNICAL SCIENCES > Biotechnology
Divisions: Division of Molecular Biology
Depositing User: Lea Vojta
Date Deposited: 06 Jun 2016 12:51
Last Modified: 06 Jun 2016 12:51
URI: http://fulir.irb.hr/id/eprint/2793
DOI: 10.1016/j.btre.2015.05.006

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