Gene expression in visceral and subcutaneous adipose tissue in overweight women

Koršić, Mirko; Gotovac, Kristina; Nikolac, Matea; Dušek, Tina; Škegro, Mate; Muck-Šeler, Dorotea; Borovečki, Fran; Pivac, Nela (2012) Gene expression in visceral and subcutaneous adipose tissue in overweight women. Frontiers in Bioscience-Elite edition, 4 . pp. 2834-2844. ISSN 1093-9946

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Abstract

Excess weight and obesity are common health problems with multifactorial and polygenic causes. Abdominal or visceral obesity is associated with a higher risk of obesity related complications. The aim of the study was to evaluate differentially expressed genes in visceral adipose tissue (VAT) and subcutaneous adipose tissue (SAT) of 10 overweight women undergoing elective laparoscopic cholecystectomy. Following expression profiling using microarrays, a set of 294 genes that exhibited differential expression between VAT and SAT was further analyzed to test the functional correlation of gene sets using the Gene Set Enrichment Analysis method. To confirm the functional pathways involved in differential expression between SAT and VAT, additional pathway analysis was done using the GeneGo MetaCore software and the Ingenuity Pathway Analysis. Ten differentially expressed genes were selected according to the microarray data, with seven exhibiting significant differential expression in the RT-PCR experiments. The data from this preliminary study suggest enrichment of inflammation and oxidative stress related pathways in VAT, while insulin homeostasis pathways as well as pathways pertaining to several growth factors are enriched in SAT.

Item Type:	Article
Uncontrolled Keywords:	obesity; differentially expressed genes; visceral adipose tissue; subcutaneous adipose tissue; functional pathways; inflammation; oxidative stress; insulin homeostasis
Subjects:	BIOMEDICINE AND HEALTHCARE > Clinical Medical Sciences
Divisions:	Division of Molecular Medicine
Projects:	Project title Project leader Project code Project type Genomic Analysis of Transcriptome and Interactome in Complex Disease Patients (Genomska analiza transkriptoma i interaktoma u bolesnika s kompleksnim bolestima)- Fran Borovečki 108-1081874-1923 MZOS Pharmacogenomics and proteomics of serotonergic and catecholaminergic system (Farmakogenomika i proteomika serotoninskog i kateholaminskog sustava)- Dorotea Muck-Šeler 098-0982522-2457 MZOS Molecular basis and treatment of psychiatric and stress related disorders (Molekularna podloga i liječenje psihijatrijskih i stresom izazvanih poremećaja)- Nela Pivac 098-0982522-2455 MZOS Differences in the genetic expression in android and gynoid obesity (Razlike u osobitostima genske ekspresije androidnog i ginoidnog tipa debljine)- Mirko Koršić 108-0000000-0162 MZOS
Depositing User:	Matea Nikolac Perković
Date Deposited:	04 Dec 2015 08:02
URI:	http://fulir.irb.hr/id/eprint/2373
DOI:	10.2741/587

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