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The Patched gene is epigenetically regulated in ovarian dermoids and fibromas, but not in basocellular carcinomas

Čretnik, Maja; Musani, Vesna; Orešković, Slavko; Leović, Dinko; Levanat, Sonja (2007) The Patched gene is epigenetically regulated in ovarian dermoids and fibromas, but not in basocellular carcinomas. International Journal of Molecular Medicine, 19 (6). pp. 875-883. ISSN 1107-3756

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The Hedgehog/Patched signaling pathway plays a prominent role during mammalian development but it is also involved in oncogenic transformation. We investigated the methylation status of the Patched promoter in a set of basocellular carcinomas of the skin and ovarian tumors as an alternative to mutational causes of the pathway deregulation. Our aim was to define a possible role of genetic and/or epigenetic mechanisms of Hedgehog/Patched signal transduction in the development of these tumors. Bisulfite-converted DNA from tumors and from matched healthy tissue was amplified by a specific PCR and the CpG-rich regions of the Patched promoter were sequenced. Two promoter regions showed statistically significant hypermethylation compared to healthy controls in ovarian tumors ; more significantly in the region in the vicinity of Gli1-binding sites and less significantly in the region containing the ATG codon. But, in basocellular carcinomas of the skin we observed no difference in methylation, suggesting different mechanisms of neoplasia in these tumors.

Item Type: Article
Uncontrolled Keywords: methylation; patched promoter; Hh-Gli signaling; tumors
Subjects: BIOMEDICINE AND HEALTHCARE > Basic Medical Sciences
Divisions: Division of Molecular Medicine
Project titleProject leaderProject codeProject type
Signal transduction in tumors: Hh-Gli interactions and therapeutic potential (Prijenos signala u tumorima: Hh-Gli put, interakcije i potencijalne terapije)-Sonja Levanat098-0982464-2461MZOS
Depositing User: Maja Sabol
Date Deposited: 27 Nov 2015 11:46
DOI: 10.3892/ijmm.19.6.875

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