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Genome-wide DNA methylation assay reveals novel candidate biomarker genes in cervical cancer

Farkas, Sanja A.; Milutin Gašperov, Nina; Grce, Magdalena; Nilsson, Torbjorn K. (2013) Genome-wide DNA methylation assay reveals novel candidate biomarker genes in cervical cancer. Epigenetics, 8 (11). pp. 1213-1225. ISSN 1559-2294

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The oncogenic human papilloma viruses (HPVs) are associated with precancerous cervical lesions and development of cervical cancer. The DNA methylation signatures of host genome in normal, precancerous and cervical cancer tissue may indicate tissue-specific perturbation in carcinogenesis. The aim of this study was to identify new candidate genes that are differentially methylated in squamous cell carcinoma compared to the DNA samples from cervical intraepithelial neoplasia grade 3 (CIN3) and normal cervical scrapes. The Illumina Infinium Human Methylation 450 K BeadChip method identifies genome-wide DNA methylation changes in CpG islands, CpG shores and shelves. Our findings showed an extensive differential methylation signature in cervical cancer compared to the CIN3 or normal cervical tissues. The identified candidate biomarker genes for cervical cancer represent several types of mechanisms in the cellular machinery that are epigenetically deregulated by hypermethylation, such as membrane receptors, intracellular signaling and gene transcription. The results also confirm the extensive hypomethylation in cancer cells of genes in the immune system. These insights into the functional role of DNA methylome alterations in cervical cancer could be clinically applicable in diagnostics and prognostics, and may guide the development of new epigenetic therapies.

Item Type: Article
Uncontrolled Keywords: DNA methylation; Infinium Human Methylation 450 BeadChip; HPV; cervical cancer; immune system
Subjects: BIOMEDICINE AND HEALTHCARE > Basic Medical Sciences
Divisions: Division of Molecular Medicine
Project titleProject leaderProject codeProject type
Aberrant DNA methylation in HPV associated lesions (Promijenjeno stanje DNA-metilacije u HPV-povezanim oštećenjima)Magdalena Grce098-0982464-2510MZOS
Depositing User: Nina Milutin Gašperov
Date Deposited: 23 Nov 2015 16:10
DOI: 10.4161/epi.26346

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